Y79视网膜母细胞瘤细胞中的神经元胰岛素受体
Neuronal insulin receptors in Y79 retinoblastoma cells.
作者信息
Law N M, Rosenzweig S A
机构信息
Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston 29425, USA.
出版信息
Biochem Biophys Res Commun. 1995 May 5;210(1):58-66. doi: 10.1006/bbrc.1995.1627.
Immunoblot analysis of Y79 cell membrane proteins indicated that Y79 insulin receptors (InsRs) alpha subunits had a mass of 115 kDa. Biosynthetic studies revealed a typical transit time for InsR delivery to the Golgi (approximately 2h) and receptor processing. However, neither the proreceptor nor the mature receptor exhibited endoglycosidase H-resistance, consistent with a lack of N-linked glycan processing. Insulin stimulated a rapid and transient tyrosine phosphorylation of receptor beta subunits (95 kDa) and of IRS-1 in intact Y79 cells, whereas in vitro studies with enriched membrane glycoproteins resulted in the autophosphorylation of both InsR (95 kDa) and IGF-1-R (98k Da) beta subunits. These studies provide the first biochemical dissection of InsR structure and function in retinoblastoma cells.
Y79细胞膜蛋白的免疫印迹分析表明，Y79胰岛素受体（InsRs）α亚基的质量为115 kDa。生物合成研究揭示了InsR转运至高尔基体的典型转运时间（约2小时）以及受体加工过程。然而，前体受体和成熟受体均未表现出对内切糖苷酶H的抗性，这与缺乏N-连接聚糖加工一致。胰岛素刺激完整Y79细胞中受体β亚基（95 kDa）和IRS-1的快速瞬时酪氨酸磷酸化，而对富集膜糖蛋白的体外研究导致InsR（95 kDa）和IGF-1-R（98 kDa）β亚基的自磷酸化。这些研究首次对视网膜母细胞瘤细胞中InsR的结构和功能进行了生化剖析。
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