Fitscher B A, Klaassen-Schlüter C M, Stremmel W
Department of Medicine IV, University Hospital of Heidelberg, Germany.
Biochim Biophys Acta. 1995 Apr 28;1256(1):47-51. doi: 10.1016/0005-2760(95)00003-u.
The aim of the present study was to directly demonstrate that hepatocellular uptake of long-chain fatty acids represents a non-diffusional uptake mechanism. Xenopus laevis oocytes were used for expression of rat liver mRNA to identify the liver fatty acid uptake system. Injection of total rat liver poly(A)+ RNA into oocytes resulted in a dose-dependent increase in fatty acid uptake. The most active mRNA was found in the 1.1-2.1 kb subfraction. In contrast, expression of the liver cytosolic fatty acid binding protein (L-FABP) or the previously suggested candidate carrier protein, mitochondrial aspartate aminotransferase (mGOT), did not induce fatty acid uptake. It is concluded that in rat liver, fatty acid transport represents a protein-mediated transport system.
本研究的目的是直接证明肝细胞对长链脂肪酸的摄取代表一种非扩散性摄取机制。非洲爪蟾卵母细胞用于表达大鼠肝脏mRNA以鉴定肝脏脂肪酸摄取系统。将大鼠肝脏总多聚腺苷酸加尾RNA注射到卵母细胞中导致脂肪酸摄取呈剂量依赖性增加。在1.1 - 2.1 kb亚组分中发现活性最高的mRNA。相比之下，肝脏胞质脂肪酸结合蛋白（L-FABP）或先前提出的候选载体蛋白线粒体天冬氨酸转氨酶（mGOT）的表达并未诱导脂肪酸摄取。得出的结论是，在大鼠肝脏中，脂肪酸转运代表一种蛋白质介导的转运系统。
