牛蛙（牛蛙）碱性非特异性酸性核糖核酸酶的纯化及性质

Purification, and properties of a base non-specific acid ribonuclease from bullfrog (Rana catesbeiana).

作者信息

Yagi H, Kobayashi H, Inokuchi N, Koyama T, Irie M

机构信息

Department of Microbiology, College of Pharmacy, Nihon University, Chiba, Japan.

出版信息

Biol Pharm Bull. 1995 Feb;18(2):219-22. doi: 10.1248/bpb.18.219.

DOI:10.1248/bpb.18.219

PMID:7742787

Abstract

An acid ribonuclease (RNase RCL2) was purified to homogeneity on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) from a homogenate of bullfrog liver (Rana catesbeiana). The apparent molecular weight estimated from SDS-PAGE was ca. 25kDa. The pH optimum of the RNase was 5.0. The RNase released mononucleotides from RNA in the order of 3'-UMP, 3'-GMP and 3'-AMP. The N-terminal amino acid sequence of RNase RCL2 was determined up to the 20th residue, and it was found to have a 5 residue sequence homology with that of oyster acid RNase [H. Watanabe et al. J. Biochem. (Tokyo), 114, 800 (1993)]. Thus, RNase RCL2 seems to be a member of the RNase T2 family RNases. This is the first evidence of the RNase T2 family RNase in amphibians.

摘要

从牛蛙（牛蛙）肝脏匀浆中通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）将一种酸性核糖核酸酶（RNase RCL2）纯化至同质。SDS - PAGE估计的表观分子量约为25kDa。该核糖核酸酶的最适pH为5.0。该核糖核酸酶从RNA中释放单核苷酸的顺序为3'-UMP、3'-GMP和3'-AMP。测定了RNase RCL2的N端氨基酸序列直至第20个残基，发现它与牡蛎酸性核糖核酸酶的序列有5个残基的同源性[H. Watanabe等人，《生物化学杂志》（东京），114，800（1993）]。因此，RNase RCL2似乎是核糖核酸酶T2家族核糖核酸酶的一员。这是两栖动物中存在核糖核酸酶T2家族核糖核酸酶的首个证据。