Loegering D J, McCormack J P, Bruner C A
Department of Physiology, Albany Medical College, New York 12208, USA.
Shock. 1994 Sep;2(3):192-5. doi: 10.1097/00024382-199409000-00006.
Previous studies have shown that the injection of IgG-coated erythrocytes (EIgG) increased the mortality rate caused by bacterial lipopolysaccharide (LPS) and that animals made tolerant to LPS show a decrease in the mortality rate caused by LPS. The present study determined the effect of the injection of EIgG and of LPS tolerance on the depression of vascular reactivity caused by LPS in vivo or in vitro. For in vivo studies, LPS was injected intravenously into rats 2 h after the injection of E or EIgG. Aortae were removed 90 min after the injection of LPS, and cumulative concentration-response curves to phenylephrine were performed in helically cut aortic strips. LPS (.1 mg/kg) caused a 21% decrease in the maximum tension developed in response to phenylephrine in aortae from animals given erythrocytes. In contrast, animals given EIgG showed a 63% decrease in maximum tension following the injection of this dose of LPS. For in vitro studies, the depression of vascular reactivity caused by incubation with LPS of aortae from rats injected with EIgG was determined. As with the in vivo studies, there was a greater depression of vascular reactivity caused by incubation with LPS of aortae taken from animals injected with EIgG. Similar studies were carried out with rats made tolerant to LPS. Tolerance was induced by giving a regimen of five daily injections of LPS. Following the injection of LPS (1 mg/kg), the maximum tension of aortae from sham tolerant animals was depressed 63%, while aortae from LPS tolerant animals were depressed only 16%.(ABSTRACT TRUNCATED AT 250 WORDS)
先前的研究表明，注射免疫球蛋白G包被的红细胞（EIgG）会增加由细菌脂多糖（LPS）导致的死亡率，并且对LPS产生耐受的动物由LPS导致的死亡率会降低。本研究确定了注射EIgG和LPS耐受对体内或体外LPS所致血管反应性降低的影响。在体内研究中，在注射E或EIgG后2小时，将LPS静脉注射到大鼠体内。在注射LPS后90分钟取出主动脉，在螺旋形切割的主动脉条上进行对去氧肾上腺素的累积浓度-反应曲线实验。LPS（0.1毫克/千克）使接受红细胞的动物主动脉中对去氧肾上腺素产生的最大张力降低了21%。相比之下，接受EIgG的动物在注射该剂量LPS后最大张力降低了63%。在体外研究中，测定了用LPS孵育注射EIgG的大鼠的主动脉所引起的血管反应性降低情况。与体内研究一样，用LPS孵育注射EIgG的动物的主动脉所引起的血管反应性降低更大。对LPS产生耐受的大鼠也进行了类似研究。通过每日注射五次LPS的方案诱导耐受。注射LPS（1毫克/千克）后，假耐受动物的主动脉最大张力降低了63%，而LPS耐受动物的主动脉仅降低了16%。（摘要截短于250字）
