Yang S, Hauptman J G
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing 48824, USA.
Shock. 1994 Dec;2(6):433-7. doi: 10.1097/00024382-199412000-00008.
Sepsis/septic shock and multiple organ failure are important causes of morbidity and mortality. Our objective was to study sepsis and organ failure in a fluid-resuscitated septic model. Males S-D rats were anesthetized with halothane, the jugular vein catheterized, and CLP performed. Each rat was maintained in a metabolism cage on continuous intravenous fluid (3 mL/rat). Urine rate and [creatinine]urine were measured daily. At day 5, serum creatinine with chemistry profile, complete blood count, clotting times, and wet lung/body weight ratios were also measured. Glomerular filtration rate (GFR) was measured according to the principle of endogenous creatinine clearance. GFR was correlated with the product of urine rate x [creatinine]urine (R = .79), so that product was used as a daily indicator of GFR. Urine output remained > or = normal during sepsis. Heparin and antithrombin III were tested in this model. The model was associated with 40% mortality, a 60% reduction in platelet count, liver damage, a 75% reduction in renal function, muscle damage, and a normal wet lung/body weight ratio. Treatment with heparin/antithrombin III ameliorated the decrease in GFR (p < .05) observed in the nontreated animals, prevented the septic-induced thrombocytopenia (p < .05), and improved survival (p = .05).
脓毒症/脓毒性休克和多器官功能衰竭是发病和死亡的重要原因。我们的目的是在液体复苏的脓毒症模型中研究脓毒症和器官功能衰竭。雄性Sprague-Dawley大鼠用氟烷麻醉，颈静脉插管，行盲肠结扎穿孔术（CLP）。每只大鼠置于代谢笼中，持续静脉输液（3 mL/只）。每天测量尿量和尿肌酐浓度。在第5天，还测量血清肌酐、生化指标、全血细胞计数、凝血时间以及湿肺/体重比。根据内生肌酐清除率原理测量肾小球滤过率（GFR）。GFR与尿量×尿肌酐浓度的乘积相关（R = 0.79），因此该乘积用作GFR的每日指标。脓毒症期间尿量保持≥正常水平。在该模型中对肝素和抗凝血酶III进行了测试。该模型的死亡率为40%，血小板计数降低60%，肝脏损伤，肾功能降低75%，肌肉损伤，湿肺/体重比正常。肝素/抗凝血酶III治疗改善了未治疗动物中观察到的GFR降低（p < 0.05），预防了脓毒症诱导的血小板减少（p < 0.05），并提高了生存率（p = 0.05）。
