O'Shaughnessy T J, Kim Y I
Department of Biomedical Engineering, University of Virginia School of Medicine, Charlottesville 22908, USA.
Comput Methods Programs Biomed. 1995 Jan;46(1):79-90. doi: 10.1016/0169-2607(94)01601-b.
The study of synaptic biopotentials at the neuromuscular junction has proven to be an invaluable tool in understanding the pre- and post-synaptic mechanisms underlying chemical synaptic transmission and exploring the pathogenesis of neuromuscular disorders. Computer technology has become an important part of these experiments as it increases accuracy and automates the process, allowing more data to be collected and quantitatively analysed. This paper presents a computer program for IBM PC compatible computers capable of recording and analysing the spontaneously occurring miniature end-plate potentials (MEPPs), neurally evoked end-plate potentials (EPPs) and muscle fiber action potentials (APs). The program performs its basic analysis immediately following the sampling of biopotentials from a muscle fiber, thus providing the user with immediate feedback on the status of the experiment; 45 parameters of neuromuscular transmission are computed on-line and displayed on the monitor along with the signal-averaged waveforms. After a series of fibers has been recorded, the program will compile the data into summary tables. The program is designed to provide an experimenter with an easy-to-use, comprehensive method of performing electrophysiological measurements and analysis at the neuromuscular junction.
事实证明，对神经肌肉接头处突触生物电位的研究是理解化学突触传递背后的突触前和突触后机制以及探索神经肌肉疾病发病机制的宝贵工具。计算机技术已成为这些实验的重要组成部分，因为它提高了准确性并使过程自动化，从而能够收集更多数据并进行定量分析。本文介绍了一种适用于IBM PC兼容计算机的计算机程序，该程序能够记录和分析自发出现的微小终板电位（MEPPs）、神经诱发的终板电位（EPPs）和肌纤维动作电位（APs）。该程序在从肌纤维采集生物电位样本后立即进行基本分析，从而为用户提供有关实验状态的即时反馈；在线计算45个神经肌肉传递参数，并与信号平均波形一起显示在监视器上。在记录了一系列纤维后，该程序将把数据编译成汇总表。该程序旨在为实验者提供一种在神经肌肉接头处进行电生理测量和分析的易于使用的综合方法。
