Dolezel J, Göhde W
Academy of Sciences of the Czech Republic, Institute of Experimental Botany, Olomouc.
Cytometry. 1995 Feb 1;19(2):103-6. doi: 10.1002/cyto.990190203.
High-resolution flow cytometry was used to analyze nuclear DNA content in dioecious plants Melandrium album and M. rubrum. Very low coefficients of variation (0.53-0.70%) of G0/1 peaks were obtained after the analysis of nuclei isolated by chopping leaf tissues. In both species, females have lower DNA content than males due to sex chromosome heteromorphism. This made it possible to discriminate peaks belonging to female and male nuclei on the same histogram and permitted unambiguous sex identification in both species. The results indicated the possibility of diagnosing sex also in other heterogametic dioecious plants. The assay described here might be interesting in any area where rapid sex identification is required at an early stage of plant development.
采用高分辨率流式细胞术分析雌雄异株植物白麦瓶草和红麦瓶草的核DNA含量。通过切碎叶片组织分离细胞核后进行分析，得到了非常低的G0/1峰变异系数（0.53 - 0.70%）。在这两个物种中，由于性染色体异型性，雌性的DNA含量低于雄性。这使得在同一直方图上能够区分属于雌性和雄性细胞核的峰，并能够明确鉴定这两个物种的性别。结果表明，在其他异配性别的雌雄异株植物中也有可能诊断性别。本文所述的检测方法在植物发育早期需要快速进行性别鉴定的任何领域可能都很有意义。
