McLandsborough L A, Cleary P P
University of Minnesota, Minneapolis 55455, USA.
FEMS Microbiol Lett. 1995 Apr 15;128(1):45-51. doi: 10.1111/j.1574-6968.1995.tb07498.x.
Several reports have shown that Streptococcus pyogenes strains which produce opacity factor (OF+) have diverged significantly from OF- serotypes. This study questions whether several surface proteins of an OF+ culture are regulated by the positive regulatory protein VirR, in a manner similar to OF- strains. Interruption of the virR region of an OF+ S. pyogenes (strain CS101, M type 49) was performed using a temperature-sensitive plasmid containing a fragment of virR. Interruption of the virR region produced cultures with undetectable amounts of M49 and ScpA proteins, and reduced the yield of FcRA protein. In addition, mutants had a significant reduction in detectable opacity factor. These results suggest that virR functions as a positive regulator of a variety of surface proteins in OF+ strains.
多项报告显示，产生透明质酸酶（OF+）的化脓性链球菌菌株与OF-血清型有显著差异。本研究探讨OF+培养物的几种表面蛋白是否像OF-菌株一样，受正调控蛋白VirR的调控。使用含有virR片段的温度敏感质粒对OF+化脓性链球菌（CS101菌株，M49型）的virR区域进行中断。virR区域的中断导致培养物中检测不到M49和ScpA蛋白，并降低了FcRA蛋白的产量。此外，突变体的可检测透明质酸酶显著减少。这些结果表明，virR在OF+菌株中作为多种表面蛋白的正调控因子发挥作用。
