Zhang Z, Wu D
Hua Xi Yi Ke Da Xue Xue Bao. 1994 Dec;25(4):388-92.
In this study, we found that methylmercury chloride inhibited the binding of 3H-QNB to muscarinic receptor of rat brain tissue in vitro with IC50 values of 0.0137 +/- 0.0037mol/L. It also decreased the densities of muscarinic receptor and affinity to 3H-QNB. In vivo test system, the rats were exposed to methylmercury chloride (ip) 0, 1.5, 2.5, 3.5 mg/kg.d on the 7-12th day of gestation. On the 7, 14 and 21st day after birth, the offsprings were killed, and the cerebrum and cerebellum were immediately dissected on ice and homogenizated. The ligand assays were carried out. The result showed the effect of inhibition on the binding to cerebrum and cerebellum with significant correlation.
在本研究中，我们发现氯化甲基汞在体外可抑制3H-QNB与大鼠脑组织毒蕈碱受体的结合，其IC50值为0.0137±0.0037mol/L。它还降低了毒蕈碱受体的密度以及对3H-QNB的亲和力。在体内试验系统中，在妊娠第7至12天，将大鼠腹腔注射0、1.5、2.5、3.5mg/kg.d的氯化甲基汞。在出生后第7、14和21天，处死后代，立即在冰上解剖大脑和小脑并匀浆。进行配体分析。结果显示对大脑和小脑结合的抑制作用具有显著相关性。
