Khan-Dawood F S, Ayyagari R R, Dawood M Y
Department of Obstetrics, Gynecology and Reproductive Sciences, University of Texas Medical School at Houston, TX 77030, USA.
Hum Reprod. 1995 Jan;10(1):193-8. doi: 10.1093/humrep/10.1.193.
We have demonstrated the presence of epidermal growth factor (EGF) and its receptors in human non-gestational corpora lutea. To determine further the characteristics of EGF receptor binding, we examined 30 human corpora lutea throughout the luteal phase and during pregnancy. Scatchard plots of EGF binding in 29 of the 30 corpora lutea were curvilinear, suggesting negative co-operativity. The mean +/- SE of the association constant Ka was (0.9 +/- 0.2) x 10(9) l/mol, the dissociation constant Kd was (2.2 +/- 0.3) x 10(-9) mol/l and the number of binding sites (Rt) was (15.8 +/- 2.1) x 10(-19) mol/micrograms protein for non-gestational corpora lutea. The Kd increased significantly in late pregnancies compared to early pregnancies (P = < 0.005), while Rt was significantly higher in term pregnancies than in either early pregnancy (P < 0.01) or the menstrual cycle (P < 0.001). Corpora lutea atretica (n = 2) and ovarian stroma (n = 6) did not show any EGF binding activity. Our findings demonstrate the presence of specific EGF receptors in human corpora lutea of both the menstrual cycle and pregnancy. The changes in EGF binding parameters in early pregnancy suggest that there may be a relationship between the role of EGF and ovarian steroidogenesis.
我们已证实在人非妊娠黄体中存在表皮生长因子（EGF）及其受体。为进一步确定EGF受体结合的特征，我们在整个黄体期及孕期检查了30个人类黄体。30个黄体中有29个的EGF结合Scatchard图呈曲线，提示负协同性。非妊娠黄体的结合常数Ka的均值±标准误为(0.9±0.2)×10⁹ l/mol，解离常数Kd为(2.2±0.3)×10⁻⁹ mol/l，结合位点数（Rt）为(15.8±2.1)×10⁻¹⁹ mol/μg蛋白质。与早孕相比，晚孕期Kd显著升高（P = < 0.005），而足月妊娠时Rt显著高于早孕（P < 0.01）或月经周期（P < 0.001）。闭锁黄体（n = 2）和卵巢基质（n = 6）未显示任何EGF结合活性。我们的研究结果证实在月经周期和孕期的人黄体中均存在特异性EGF受体。早孕时EGF结合参数的变化提示EGF的作用与卵巢甾体生成之间可能存在关联。
