[免疫层析法检测乙型肝炎表面抗原（HBsAg）抗体的技术评估]

[Evaluation of immunochromatography assay technique for detection of antibody to hepatitis B surface antigen (HBsAg)].

作者信息

Kashiwagi S, Hayashi J, Kakuda K, Yamaji K, Ueno K, Tani Y

机构信息

Department of General Medicine, Kyushu University Hospital.

出版信息

Kansenshogaku Zasshi. 1995 Mar;69(3):297-302. doi: 10.11150/kansenshogakuzasshi1970.69.297.

DOI:10.11150/kansenshogakuzasshi1970.69.297

PMID:7745307

Abstract

A new immunochromatography assay (Dainascreen Ausab Dainabot) has been recently introduced for the detection of the presence of antibody to HBsAg. To evaluate the feasibility of using the Dainascreen Ausab, we carried out comparison tests with this method and PHA. In the test of 439 sera from HB vaccinees, inhabitants in Iki Island, Nagasaki Pref., patients with autoimmune diseases and with acute hepatitis B, 154 (31.2%) were positive by Dainascreen Ausab, 145 (29.4%) were positive by PHA and 145 (29.4%) were positive by both Dainascreen Ausab and PHA. Nine (1.8%) were positive by only Dainascreen and there were none positive by only PHA. A good correlation was observed between the titer of the antibody by this method and IMx. The anti-HBs assay by this method was able to be completed within 15 minutes and the procedure was very simple. The results indicate that the sensitivity of Dainascreen is superior to PHA and that it is easy to use.

摘要

最近推出了一种新的免疫层析检测法（Dainascreen Ausab Dainabot）用于检测乙型肝炎表面抗原（HBsAg）抗体的存在。为评估使用Dainascreen Ausab的可行性，我们用该方法与PHA进行了对比试验。在对439份血清的检测中，这些血清来自乙肝疫苗接种者、长崎县壹岐岛居民、自身免疫性疾病患者和急性乙型肝炎患者，Dainascreen Ausab检测呈阳性的有154份（31.2%），PHA检测呈阳性的有145份（29.4%），Dainascreen Ausab和PHA检测均呈阳性的有145份（29.4%）。仅Dainascreen检测呈阳性的有9份（1.8%），仅PHA检测呈阳性的无。该方法检测的抗体效价与IMx之间存在良好相关性。用该方法检测抗-HBs能够在15分钟内完成，且操作非常简单。结果表明Dainascreen的灵敏度优于PHA，且易于使用。