源自人游离龈和附着龈的成纤维细胞的异质性。糖胺聚糖合成及苯妥英（PHT）治疗的影响。

Heterogeneity of fibroblasts derived from human free and attached gingiva. Glycosaminoglycan synthesis and effects of phenytoin (PHT) treatment.

作者信息

Pagliarini A, Stabellini G, Carinci F, Calura G, Tognon M, Evangelisti R

机构信息

Dentistry Clinic, University of Ferrara, Italy.

出版信息

J Oral Pathol Med. 1995 Feb;24(2):72-7. doi: 10.1111/j.1600-0714.1995.tb01142.x.

DOI:10.1111/j.1600-0714.1995.tb01142.x

PMID:7745545

Abstract

Two fibroblast populations derived from free and attached gingiva (FGF, AGF) have been compared in cell culture. They exhibited the same morphology and similar cytoskeletal staining patterns, but were different in glycosaminoglycan (GAG) synthesis. FGF released larger quantities of GAGs than AGF in the medium. The extracellular accumulation of hyaluronic acid was higher in FGF than in AGF, whilst the reverse pattern was observed intracellularly. In the case of sulphated GAGs the extracellular concentration, compared to HA, was higher in AGF, while the intracellular concentration was higher in FGF. The two cell populations responded differently to phenytoin (PHT) administration. PHT treatment increased the proportion of intracellular sulphated GAGs in AGF and of extracellular sulphated GAGs in FGF.

摘要

对源自游离龈和附着龈的两种成纤维细胞群体（游离龈成纤维细胞、附着龈成纤维细胞）进行了细胞培养比较。它们呈现出相同的形态和相似的细胞骨架染色模式，但在糖胺聚糖（GAG）合成方面存在差异。游离龈成纤维细胞在培养基中释放的GAGs数量比附着龈成纤维细胞多。透明质酸的细胞外积累在游离龈成纤维细胞中高于附着龈成纤维细胞，而在细胞内则观察到相反的模式。就硫酸化GAGs而言，与透明质酸相比，附着龈成纤维细胞的细胞外浓度更高，而游离龈成纤维细胞的细胞内浓度更高。这两种细胞群体对苯妥英（PHT）给药的反应不同。PHT处理增加了附着龈成纤维细胞中细胞内硫酸化GAGs的比例以及游离龈成纤维细胞中细胞外硫酸化GAGs的比例。