针对癣病的比奥韦塔疫苗株TV-M-310的生长、孢子形成能力及毒力研究。

Studies on growth, spore-forming ability and virulence of the vaccine strain TV-M-310 of the vaccine Bioveta against ringworm.

作者信息

Siesenop U, Böhm K H, Brandebusemeyer E, Conrad P

机构信息

Institut für Mikrobiologie und Tierseuchen, Tierärztlichen Hochschule Hannover, Germany.

出版信息

Mycoses. 1994 Sep-Oct;37(9-10):371-6. doi: 10.1111/myc.1994.37.9-10.371.

DOI:10.1111/myc.1994.37.9-10.371

PMID:7746299

Abstract

We determined the intensity of growth, the ability to form spores and virulence in guinea pigs of the vaccine strain of the Czech live vaccine Bioveta. Comparative investigations were carried out with the vaccine strain of the Russian live vaccine LTF 130 and Trichophyton verrucosum field strains. Growth of the vaccine strains was faster than growth of the field strains on solid media and in broth. Later the vaccine strains formed many microconidia. In experimentally infected guinea pigs the virulence of the vaccine strain Bioveta was highly dependent on the infectious dose. At 10(1) CFU ml-1 (according to the number of CFUs in the Russian live vaccine) the vaccine strain Bioveta was nearly avirulent. Resuspended according to the manufacturer's instructions (at least 10(10) CFU ml-1) virulence of the vaccine strain Bioveta could be observed.

摘要

我们测定了捷克活疫苗Bioveta的疫苗株在豚鼠体内的生长强度、形成孢子的能力和毒力。与俄罗斯活疫苗LTF 130的疫苗株和疣状毛癣菌田间菌株进行了比较研究。在固体培养基和肉汤中，疫苗株的生长速度比田间菌株快。随后，疫苗株形成了许多小分生孢子。在实验感染的豚鼠中，Bioveta疫苗株的毒力高度依赖于感染剂量。在10(1) CFU/ml（根据俄罗斯活疫苗中的CFU数量）时，Bioveta疫苗株几乎无毒。按照制造商的说明重新悬浮（至少10(10) CFU/ml），可以观察到Bioveta疫苗株的毒力。