Tawill S A, Kipp W, Lucius R, Gallin M, Erttmann K D, Büttner D W
Bernhard Nocht Institute for Tropical Medicine, Hamburg, Federal Republic of Germany.
Trans R Soc Trop Med Hyg. 1995 Jan-Feb;89(1):51-4. doi: 10.1016/0035-9203(95)90656-8.
A total detergent-soluble extract of adult female Onchocerca volvulus (OvAg) and a recombinant O. volvulus protein (Ov33) linked to glutathione-S-transferase (GST) were compared with regard to their serodiagnostic suitability for differentiating between O. volvulus and Mansonella perstans infections in a region endemic for both filarial worms in western Uganda. Using OvAg in an enzyme-linked immunosorbent assay (ELISA), 98.8% sensitivity was obtained examining 84 O. volvulus microfilariae (mf) carriers living in the hyperendemic area. However, 5 of 18 (28%) sera from M. perstans mf carriers without O. volvulus mf, from another area hypoendemic for O. volvulus, cross-reacted with OvAg. Using the recombinant antigen Ov33-GST in an ELISA and Western blot assay, sensitivity for O. volvulus remained high (97.2% and 98.8% respectively) while none of 90 sera from M. perstans mf carriers reacted positively. Both antigens were used to examine a batch of sera from 260 persons living in the onchocerciasis hyperendemic area who did not have mf in their skin snips (9.5% of 2728 persons examined); 116 of these sera (44.6%) were positive in the OvAg ELISA, compared to 85 (32.7%) and 69 (26.5%) which were positive in Ov33-GST ELISA and Ov33-GST Western blot, respectively. Reaction with GST alone was minimal. The recombinant antigen Ov33 efficiently differentiates between O. volvulus and M. perstans infections, and is sensitive when used to detect patent and prepatent or low-level O. volvulus infections.
对成年雌性盘尾丝虫的全去污剂可溶性提取物（OvAg）和与谷胱甘肽-S-转移酶（GST）连接的重组盘尾丝虫蛋白（Ov33）在乌干达西部两种丝虫均为地方流行区区分盘尾丝虫和常现曼森线虫感染的血清学诊断适用性进行了比较。在酶联免疫吸附测定（ELISA）中使用OvAg，检测84名生活在高度流行区的盘尾丝虫微丝蚴（mf）携带者时，敏感性为98.8%。然而，来自另一个盘尾丝虫低度流行区的18名无盘尾丝虫mf的常现曼森线虫mf携带者的血清中有5份（28%）与OvAg发生交叉反应。在ELISA和蛋白质印迹分析中使用重组抗原Ov33-GST时，对盘尾丝虫的敏感性仍然很高（分别为97.2%和98.8%），而90名常现曼森线虫mf携带者的血清中无一呈阳性反应。两种抗原均用于检测一批来自盘尾丝虫病高度流行区的260人的血清，这些人皮肤切片中无mf（占检查的2728人的9.5%）；这些血清中有116份（44.6%）在OvAg ELISA中呈阳性，相比之下，在Ov33-GST ELISA和Ov33-GST蛋白质印迹中呈阳性的分别为85份（32.7%）和69份（26.5%）。单独与GST的反应极小。重组抗原Ov33能有效区分盘尾丝虫和常现曼森线虫感染，用于检测盘尾丝虫的显性感染、潜伏期感染或低度感染时具有敏感性。
