Gegelashvili G, Schousboe A, Linnemann D
Sarajishvili Institute of Clinical and Experimental Neurology, Tbilisi, Republic of Georgia.
Int J Dev Neurosci. 1994 Dec;12(8):703-8. doi: 10.1016/0736-5748(94)90050-7.
Cell type-specific and developmental patterns of APP expression were investigated in rat brain and cultured neural cells. Nearly all astrocytes were APP-positive, whereas only selected population of neurons appeared to express APP. In these neurons, APP immunoreactivity was preferentially restricted to single processes. mRNAs encoding the major APP isoforms, APP695 and APP770, were co-expressed as 3.4-3.6 kb transcripts both in astrocytes and neurons. In addition, an unusual 2.8 kb mRNA size class was revealed in cultured cerebellar granule neurons by means of the probe recognizing APP770 mRNA. Also, for the first time, APP714 mRNA was detected in rat brain by northern blotting. The steady-state levels of these transcripts were increased from birth up to postnatal day 20, whereas no apparent changes were observed after reaching adulthood. These data hint at the involvement of APP in the major morphogenetic events taking place in rat brain during the first three postnatal weeks.
在大鼠脑和培养的神经细胞中研究了APP表达的细胞类型特异性和发育模式。几乎所有星形胶质细胞都是APP阳性的，而只有部分特定的神经元群体似乎表达APP。在这些神经元中，APP免疫反应性优先局限于单个突起。编码主要APP异构体APP695和APP770的mRNA在星形胶质细胞和神经元中均作为3.4 - 3.6 kb的转录本共同表达。此外，通过识别APP770 mRNA的探针在培养的小脑颗粒神经元中发现了一种异常的2.8 kb mRNA大小类别。同样，通过Northern印迹法首次在大鼠脑中检测到APP714 mRNA。这些转录本的稳态水平从出生到出生后第20天升高，而成年后未观察到明显变化。这些数据提示APP参与了出生后前三周大鼠脑中发生的主要形态发生事件。
