Patkin E L, Kustova M E, Noniashvili E M
Department of Molecular Genetics, Institute for Experimental Medicine, Russian Academy of Medical Sciences, St Petersburg.
Cytobios. 1994;79(319):235-40.
The nick translation procedure without external addition of nicking enzymes was performed in situ on fixed nuclei of mouse preimplantation, and postimplantation embryos, as well as bone marrow in order to detect possible DNA single-strand breaks. All preparations of nuclei were made using the same technique. Nick translation of nuclear DNA with DNA polymerase I in the presence of biotinylated-dUTP demonstrated a characteristic absence of label on nuclei of postimplantation embryos and bone marrow. The nuclear reactivity varied according to the cleavage divisions of the zygote, being highest at the four-cell stage.
在不额外添加切口酶的情况下，对小鼠植入前、植入后胚胎的固定细胞核以及骨髓进行原位切口平移操作，以检测可能存在的DNA单链断裂。所有细胞核标本均采用相同技术制备。在生物素化dUTP存在的情况下，用DNA聚合酶I对细胞核DNA进行切口平移，结果显示植入后胚胎和骨髓细胞核上无标记。细胞核反应性根据合子的卵裂情况而变化，在四细胞期最高。
