Bergamaschi S, Govoni S, Battaini F, Parenti M, Trabucchi M
Institute of Pharmacological Sciences, University of Milano, Italy.
Alcohol Alcohol Suppl. 1993;2:403-7.
Chronic ethanol exposure alters calcium movements and the number of dihydropyridine (DHP) binding sites in the animal brain and in cultured neural cell lines. The present study investigates the effect of ethanol treatment on dihydropyridine- and omega-conotoxin (CgTx)- labelled calcium channels in undifferentiated and differentiated NG 108-15 cells. Following differentiation, the binding of [3H] -PN 200-110 increased by 48% while [125I]-CgTx binding increased by six fold. In accordance with data in the literature, undifferentiated cells exposed to ethanol (3 days) showed a large increase (70%) in DHP binding sites. In contrast, CgTx binding sites were unmodified by the treatment. Using differentiated cells, ethanol exposure induced a significant decrease (53%) in CgTx binding sites without altering those labelled by PN 200-110 (isradipine). These findings indicate that the effect of ethanol exposure on calcium channels may be dependent on the type of channel considered and on the status of the cells treated.
长期乙醇暴露会改变动物大脑和培养的神经细胞系中的钙运动以及二氢吡啶（DHP）结合位点的数量。本研究调查了乙醇处理对未分化和分化的NG 108 - 15细胞中用二氢吡啶和ω-芋螺毒素（CgTx）标记的钙通道的影响。分化后，[3H] - PN 200 - 110的结合增加了48%，而[125I] - CgTx结合增加了六倍。与文献数据一致，暴露于乙醇（3天）的未分化细胞的DHP结合位点大幅增加（70%）。相反，CgTx结合位点未因该处理而改变。使用分化细胞时，乙醇暴露导致CgTx结合位点显著减少（53%），而未改变由PN 200 - 110（伊拉地平）标记的位点。这些发现表明，乙醇暴露对钙通道的影响可能取决于所考虑的通道类型以及所处理细胞的状态。
