File S E, Andrews N, al-Farhan M, Wu P Y
Psychopharmacology Research Unit, Guy's Hospital, London, U.K.
Alcohol Alcohol Suppl. 1993;2:495-9.
There was a significant increase in potassium-stimulated release of 3H-[5-HT] from hippocampal slices taken from rats withdrawn from chronic ethanol treatment, compared with control-treated rats. The anxiogenic behaviour observed 12 h after ethanol withdrawal was inhibited by the 5-HT1A partial agonist, buspirone (200 micrograms/kg s.c.), indicating that the increased 5-HT release might underlie the anxiogenic response. The ex-ethanol treated rats showed impaired habituation of motor activity in the holeboard and a reduced exploratory response. The latter, but not the former, were reversed by the 5-HT3 receptor antagonist, ondansetron (0.01 microgram/kg i.p.). Ondansetron was without effect on working memory errors, but significantly increased the number of reference memory errors made by the ex-ethanol group. It also had a significantly anxiogenic effect in this group. These results suggest that the chronic ethanol treatment changes the 5-HT system and has long-lasting effects on the function of 5-HT3 receptors.
与对照处理的大鼠相比，从慢性乙醇处理后戒断的大鼠获取的海马切片中，钾刺激的3H-[5-羟色胺]释放显著增加。乙醇戒断12小时后观察到的致焦虑行为被5-HT1A部分激动剂丁螺环酮（200微克/千克，皮下注射）抑制，这表明5-羟色胺释放增加可能是致焦虑反应的基础。经乙醇处理的大鼠在穿洞板试验中运动活动的习惯化受损，探索反应减少。5-HT3受体拮抗剂昂丹司琼（0.01微克/千克，腹腔注射）可逆转后者，但不能逆转前者。昂丹司琼对工作记忆错误没有影响，但显著增加了经乙醇处理组的参考记忆错误数量。在该组中它也有显著的致焦虑作用。这些结果表明，慢性乙醇处理改变了5-羟色胺系统，并对5-HT3受体的功能有长期影响。
