Ruano D, Lambolez B, Rossier J, Paternain A V, Lerma J
Institut Alfred Fessard, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
Neuron. 1995 May;14(5):1009-17. doi: 10.1016/0896-6273(95)90339-9.
To determine the kainate receptor subunits that are found in native kainate receptors, we have applied a multiplex PCR of cDNAs reverse transcribed from mRNA harvested from single cultured hippocampal neurons after electrophysiological recording. We found that all the cells showing rapidly desensitizing currents in response to kainate express the GluR6 subunit mRNA, and that some of them also express the GluR5 subunit mRNA. No GluR7, KA-1, or KA-2 subunit mRNAs were detected. Analysis of the editing sites of the GluR6 mRNA demonstrated that the three editing sites present in these subunits are edited to a different extent. Predominant expression of the unedited variant (Q) was observed, but edited and unedited variants may coexist in the same cell. In addition, we show that the Q/R site from the GluR6 subunit controls functional properties of native kainate receptors.
为了确定天然海人酸受体中存在的海人酸受体亚基，我们对从单个培养的海马神经元中收获的mRNA反转录得到的cDNA进行了多重PCR，这些神经元在电生理记录后被采集。我们发现，所有对海人酸产生快速脱敏电流的细胞都表达GluR6亚基mRNA，其中一些细胞还表达GluR5亚基mRNA。未检测到GluR7、KA-1或KA-2亚基mRNA。对GluR6 mRNA编辑位点的分析表明，这些亚基中存在的三个编辑位点的编辑程度不同。观察到未编辑变体（Q）的主要表达，但编辑和未编辑变体可能在同一细胞中共存。此外，我们表明，GluR6亚基的Q/R位点控制天然海人酸受体的功能特性。
