Korting H C, Stolz W, Schmid M H, Maierhofer G
Department of Dermatology, Ludwig-Maximilians-University, München, Germany.
Br J Dermatol. 1995 Apr;132(4):571-9. doi: 10.1111/j.1365-2133.1995.tb08713.x.
Upon topical application of liposomes of the large unilamellar vesicle type to human epidermis reconstructed in vitro, there is a dose-dependent alteration of the morphology of both the stratum corneum and the living part of the epidermis. In particular, shrunken lipid droplets are found between corneocytes and keratinocytes. Sometimes, corneocytes show inclusions reminiscent of 'cholesterol crystals'. Corneocytes, moreover, show a decreased density. Both corneocytes of the various layers of the stratum corneum and keratinocytes belonging to the uppermost layer of the living epidermis show particularly osmophilic membranes, indicating lipid transfer. Intact liposomes or their remnants can sometimes be seen between corneocytes of the upper strata. The presence of liposomal lipid within the stratum corneum is supported by the presence of gold particles used as a marker. There is, however, no evidence for the uptake of intact liposomes by the living epidermis, or their passage through this compartment of the skin.
将大单层囊泡类型的脂质体局部应用于体外重建的人表皮时，角质层和表皮活性部分的形态会发生剂量依赖性改变。特别是，在角质形成细胞和角质形成细胞之间发现了收缩的脂滴。有时，角质形成细胞显示出类似于“胆固醇晶体”的内含物。此外，角质形成细胞的密度降低。角质层各层的角质形成细胞和属于活性表皮最上层的角质形成细胞均显示出特别嗜渗的膜，表明存在脂质转移。有时可以在上层角质形成细胞之间看到完整的脂质体或其残余物。用作标记的金颗粒的存在支持了角质层中脂质体脂质的存在。然而，没有证据表明活性表皮摄取了完整的脂质体，或者它们穿过了皮肤的这一区域。
