Roth A, Urmoneit B, Messer W
Max-Planck-Institut für molekulare Genetik, Berlin-Dahlem, Germany.
Biochimie. 1994;76(10-11):917-23. doi: 10.1016/0300-9084(94)90016-7.
Using methidiumpropyl-EDTA (MPE) footprinting we found one specific binding site for FIS protein in the E coli replication origin, oriC. We mutagenized the binding sites for FIS and IHF in oriC and analyzed the effect of the mutations on protein binding and oriC function. The replication efficiency of oriC plasmids paralleled the ability of the mutated DNA fragments to bind IHF or FIS. We conclude that these histone-like proteins function in cis in the initiation of DNA replication at oriC.
使用甲磺酸丙基乙二胺四乙酸（MPE）足迹法，我们在大肠杆菌复制起点oriC中发现了一个FIS蛋白的特异性结合位点。我们对oriC中FIS和IHF的结合位点进行了诱变，并分析了这些突变对蛋白质结合和oriC功能的影响。oriC质粒的复制效率与突变DNA片段结合IHF或FIS的能力平行。我们得出结论，这些组蛋白样蛋白在oriC处DNA复制起始过程中以顺式发挥作用。
