Loke W K, Khor E
Department of Chemistry, National University of Singapore.
Biomaterials. 1995 Feb;16(3):251-8. doi: 10.1016/0142-9612(95)92125-p.
Shrinkage temperature is most often used to report the degree of cross-linking in glutaraldehyde-fixed animal tissue for use in bioprosthetic heart valve fabrication. Present practice utilizes the measurement of hydrothermal shrinkage observed when a sample is subjected to a temperature programme. This measurement at best gives a general indication of the efficiency of the treatment, i.e. the extent of cross-linking in the tissue. When differential scanning calorimetry has been used, the ambiguity arising from the scant reporting of the protocols used does not permit easy comparison of experimental results. This report addresses the considerations necessary to obtain optimum results in the differential scanning calorimetry experiment for the determination of shrinkage temperature in biological tissue. The shrinkage temperature of two previously unreported tissue types, porcine pericardium and equine pericardium, are provided and compared with those of bovine pericardium and porcine aortic valve leaflets.
收缩温度最常用于报告戊二醛固定的动物组织中的交联程度，该组织用于生物人工心脏瓣膜制造。目前的做法是利用对样品进行温度程序时观察到的热液收缩测量。这种测量充其量只能给出处理效率的大致指示，即组织中的交联程度。当使用差示扫描量热法时，由于所使用的实验方案报告不足而产生的模糊性使得实验结果难以轻松比较。本报告阐述了在差示扫描量热法实验中获得最佳结果以测定生物组织收缩温度所需考虑的因素。提供了两种先前未报告的组织类型（猪心包和马心包）的收缩温度，并与牛心包和猪主动脉瓣小叶的收缩温度进行了比较。
