Maynadié M, Lizard G, Solary E, Parmeland C, Carli P M
Laboratoire d'hématologie, CHRU de Dijon, France.
Bull Cancer. 1994 May;81(5):409-13.
The neoplasias resistance to chemotherapy is mainly due to multidrug resistance phenomenon (MDR) mediated by an ATP-dependent efflux pump called P-glycoprotein. This function can be reversed by many multidrug reversing agents so numerous chemotherapy regimens have been initiated in malignancies. To make sure the success of these protocols it is necessary to detect as soon and as certain as possible the presence of resistant cells among malignant population. We have chosen to evaluate functional test using rhodamine 123 in this aim in view. We have made various mixtures of resistant ans sensitive cells of three cell lines. Rhodamine 123 allows to detect 1% of resistant cells among sensitive cells. Influence of dead cells on the interpretation is discussed.
肿瘤对化疗的耐药性主要归因于一种名为P-糖蛋白的ATP依赖性外排泵介导的多药耐药现象（MDR）。许多多药逆转剂可以逆转这种功能，因此在恶性肿瘤中已经启动了许多化疗方案。为确保这些方案的成功，有必要尽早且尽可能确定地检测恶性细胞群体中耐药细胞的存在。出于这个目的，我们选择使用罗丹明123评估功能测试。我们制备了三种细胞系的耐药细胞和敏感细胞的各种混合物。罗丹明123能够检测出敏感细胞中1%的耐药细胞。讨论了死细胞对结果解读的影响。
