植物血凝素和内毒素增强大鼠对同基因格罗斯病毒诱导淋巴瘤的细胞介导细胞毒性作用。

Augmentation of cell-mediated cytotoxicity against syngeneic Gross virus-induced lymphoma in rats by phytohemagglutinin and endotoxin.

作者信息

Glaser M, Djeu J Y, Kirchner H, Herberman R B

出版信息

J Immunol. 1976 Jun;116(6):1512-9.

Abstract

Normal W/Fu rat spleen cells cultured for 1 day with mitogenic concentrations of phytohemagglutinin (PHA) or with a wide range of concentrations of endotoxin (LPS) exerted cytotoxic activity against the syngeneic Gross virus-induced lymphoma, (C58NT)D, and also against other tumor cells in a 4-hr 51Cr release assay. This activity did not involve release of detectable lymphotoxins, nor did it require the presence of additional mitogen in the media during the cytotoxicity assay. The cytotoxic activity induced by PHA was dependent on the presence of T cells while the activity induced by LPS was not dependent on T cells and appeared to require the presence of macrophages. Both PHA and LPS also augmented the cytotoxic activity of spleen cells immune against (C58NT)D tumor cells. With submitogenic concentrations of PHA, only specific cytotoxicity against (C58NT)D cells was augmented. In contrast, LPS and mitogenic concentrations of PHA caused nonspecific augmentation. Mitogenic concentrations of PHA and LPS also augmented the antibody-dependent cytotoxicity of normal W/Fu rat spleen cells against (C58NT)D cells in the presence of syngeneic anti-serum.

摘要

正常W/Fu大鼠脾细胞在有丝分裂原浓度的植物血凝素（PHA）或多种浓度的内毒素（LPS）作用下培养1天，在4小时的51Cr释放试验中对同基因格罗斯病毒诱导的淋巴瘤（C58NT）D以及其他肿瘤细胞表现出细胞毒性活性。这种活性不涉及可检测到的淋巴毒素的释放，在细胞毒性试验期间培养基中也不需要额外的有丝分裂原存在。PHA诱导的细胞毒性活性依赖于T细胞的存在，而LPS诱导的活性不依赖于T细胞，似乎需要巨噬细胞的存在。PHA和LPS都增强了免疫（C58NT）D肿瘤细胞的脾细胞的细胞毒性活性。使用亚有丝分裂原浓度的PHA时，仅增强了对（C58NT）D细胞的特异性细胞毒性。相反，LPS和有丝分裂原浓度的PHA引起非特异性增强。有丝分裂原浓度的PHA和LPS在同基因抗血清存在的情况下也增强了正常W/Fu大鼠脾细胞对（C58NT）D细胞的抗体依赖性细胞毒性。