Osaka M, Matsuo S, Koh T, Liang P, Kinoshita H, Maeda S, Sugiyama T
Department of Pathology, Faculty of Medicine, Kyoto University, Japan.
Cancer Lett. 1995 May 4;91(1):25-31. doi: 10.1016/0304-3835(94)03714-t.
Intravenous injections of 7,12-dimethylbenz[a]anthracene (DMBA) induce erythroblastic leukemia (erythroleukemia) with No.2 trisomy in Long-Evans rats. Activation of some oncogenes such as abl and Ha-ras has been reported to occur in relation to the secondary chromosomal translocations. In the present studies, a consistent type of mutation, A to T transversion in codon 61 of N-ras gene, was found in all of 6 cultured leukemia cell lines and 5 primary leukemias induced by DMBA. The N-ras mutation was also found in bone marrow cells of 2 out of 8 preleukemias. On the contrary, no mutation was observed in Ha- and Ki-ras genes in all leukemias and preleukemias. The consistent occurrence of above N-ras mutation in leukemias indicates that it plays an important role in DMBA-leukemogenesis.
静脉注射7,12-二甲基苯并[a]蒽（DMBA）可在长-伊文斯大鼠中诱发具有2号三体的成红细胞白血病（红白血病）。据报道，一些癌基因如abl和Ha-ras的激活与继发性染色体易位有关。在本研究中，在6个培养的白血病细胞系和5个由DMBA诱导的原发性白血病中，均发现了一种一致的突变类型，即N-ras基因第61密码子由A到T的颠换。在8个白血病前期中的2个的骨髓细胞中也发现了N-ras突变。相反，在所有白血病和白血病前期中，未观察到Ha-和Ki-ras基因的突变。白血病中上述N-ras突变的一致出现表明它在DMBA诱导的白血病发生中起重要作用。
