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转化生长因子β1诱导胎鼠棕色脂肪细胞中分化特异性基因表达。

Transforming growth factor beta 1 induces differentiation-specific gene expression in fetal rat brown adipocytes.

作者信息

Teruel T, Valverde A M, Benito M, Lorenzo M

机构信息

Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.

出版信息

FEBS Lett. 1995 May 8;364(2):193-7. doi: 10.1016/0014-5793(95)00385-m.

DOI:10.1016/0014-5793(95)00385-m

Abstract

Fetal rat brown adipocytes show a low number of transforming growth factor beta 1 (TGF-beta 1) binding sites of high affinity, revealing the presence of type I, II and III TGF-beta 1 receptors and a minor-labeled species of approximately 140 kDa. The culture of cells in the presence of TGF-beta 1 induced the expression of the tissue-specific gene uncoupling protein in a dose- and time-dependent manner. In addition, TGF-beta 1 up-regulates the expression of genes involved in adipogenesis such as fatty acid synthase, glycerol 3-phosphate dehydrogenase, malic enzyme and glucose 6-phosphate dehydrogenase, as well as induces the expression of fibronectin (specific target gene for TGF-beta 1). Our results suggest that TGF-beta 1 is a major signal involved in initiating and/or maintaining the thermogenic and adipogenic differentiation of rat fetal brown adipocytes.

摘要

胎鼠棕色脂肪细胞显示出数量较少但亲和力高的转化生长因子β1（TGF-β1）结合位点，揭示了I型、II型和III型TGF-β1受体的存在以及一种约140 kDa的少量标记物种。在TGF-β1存在的情况下培养细胞，以剂量和时间依赖性方式诱导了组织特异性基因解偶联蛋白的表达。此外，TGF-β1上调参与脂肪生成的基因表达，如脂肪酸合酶、甘油3-磷酸脱氢酶、苹果酸酶和葡萄糖6-磷酸脱氢酶，同时诱导纤连蛋白（TGF-β1的特异性靶基因）的表达。我们的结果表明，TGF-β1是参与启动和/或维持大鼠胎儿棕色脂肪细胞产热和脂肪生成分化的主要信号。

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