Huang J C, Khan-Dawood F S, Dawood M Y, Yeh J
Harvard Medical School, Boston, Massachusetts, USA.
Fertil Steril. 1995 Jun;63(6):1318-21.
To determine the presence and compare the relative abundance of messenger RNA for epidermal growth factor receptor (EGFR) in baboon corpora lutea of different luteal ages.
Prospective controlled nonhuman primate study.
Animal facility in an academic research institution.
Six adult female baboons with well-defined regular menstrual cycles. Stage of the menstrual cycle was determined by observation and scoring of perineal turgescence. The day of maximal turgescence was referred to as the day of LH surge.
Ten corpora lutea were obtained by luteectomy during early (LH + 1 to 5 days, n = 3), midluteal (LH + 6 to 10 days, n = 3), and late (LH + 11 to 15 days, n = 4) luteal phases.
Relative levels of messenger RNA for EGFR as determined by ribonuclease protection assay using RNA probe generated from complementary DNA for human EGFR.
Messenger RNA for EGFR is present in baboon corpora lutea with relative levels of 0.51 +/- 0.18 (mean +/- SEM) in the early, 0.43 +/- 0.17 in the midluteal, and 0.50 +/- 0.17 arbitrary units in the late luteal phase.
Baboon corpus luteum is a site of EGFR production; the levels of its messenger RNA did not change appreciably throughout the luteal phase.
确定不同黄体期狒狒黄体中表皮生长因子受体（EGFR）信使核糖核酸（mRNA）的存在情况，并比较其相对丰度。
前瞻性对照非人灵长类动物研究。
一所学术研究机构的动物设施。
6只成年雌性狒狒，月经周期规律且明确。通过观察会阴肿胀情况并评分来确定月经周期阶段。肿胀最明显的那天被称为促黄体生成素（LH）高峰日。
在黄体早期（LH + 1至5天，n = 3）、黄体中期（LH + 6至10天，n = 3）和黄体晚期（LH + 11至15天，n = 4），通过黄体切除术获取10个黄体。
使用从人EGFR互补DNA生成的RNA探针，通过核糖核酸酶保护试验确定EGFR信使核糖核酸的相对水平。
狒狒黄体中存在EGFR信使核糖核酸，在黄体早期相对水平为0.51±0.18（平均值±标准误），黄体中期为0.43±0.17，黄体晚期为0.50±0.17任意单位。
狒狒黄体是EGFR产生的部位；其信使核糖核酸水平在整个黄体期没有明显变化。
