Fu S J, Fan C C, Song H W, Wei F Q
Department of Forensic Chemistry, China Medical University, Shenyang.
Forensic Sci Int. 1995 May 9;73(1):35-40. doi: 10.1016/0379-0738(95)01709-7.
Diastereomeric dipeptides were derived from the amino acid enantiomers in dentin by O-phthalaldehyde-N-acetyl-L-cysteine. The products were separated using high performance liquid chromatography (HPLC) and detected by fluorescence detector. A short analysis time (total analysis time was 15 min, including retention time, sample derivatization time and column regeneration time) was used. The sensitivity of detection was 1 pmol and high resolution (Rs = 1.5) was reached. We determined the D/L ratio of aspartic acid in dentin of 28 first premolars. The correlation value between the D/L ratio of aspartic acid and actual age was 0.9887; errors of +/- 1 year accounted for 46.4%, and no error exceeded 5 years.
通过邻苯二甲醛-N-乙酰-L-半胱氨酸从牙本质中的氨基酸对映体衍生出非对映体二肽。产物使用高效液相色谱（HPLC）进行分离，并通过荧光检测器进行检测。采用了较短的分析时间（总分析时间为15分钟，包括保留时间、样品衍生化时间和柱再生时间）。检测灵敏度为1皮摩尔，达到了高分辨率（Rs = 1.5）。我们测定了28颗第一前磨牙牙本质中天冬氨酸的D/L比值。天冬氨酸D/L比值与实际年龄之间的相关值为0.9887；±1岁的误差占46.4%，且无误差超过5年。
