Yang W P, Oshida Y, Wu W, Sato J, Ohsawa I, Sato Y
Research Center of Health, Physical Fitness and Sports, Nagoya University, Japan.
Int J Sports Med. 1995 Feb;16(2):99-104. doi: 10.1055/s-2007-972973.
The effect of physical training on in vivo insulin-stimulated glucose utilization in relation to glycolysis (lactate formation) in rat peripheral tissues was investigated in 8 sedentary controls (SC) and 7 voluntary running rats (VR). We used a sequential euglycemic clamp procedure (insulin infusion rate; 6.0, 30.0 mU/kg.min) in combination with a microdialysis technique in M. quadriceps femoris, vastus lateralis, and inguinal adipose tissue. In the clamp study, glucose infusion rate (GIR) averaged over 45-75 min during the 6.0-mU/kg.min insulin infusion was significantly (p < 0.01) higher in VR (15.36 +/- 0.83 mg/kg.min, mean +/- SE) than in SC (10.41 +/- 0.88 mg/kg.min), and the lack of a significant difference in GIR between VR and SC was found during the 30.0-mU/kg.min insulin infusion. In these tissues, there was no significant difference in dialysate lactate levels between VR and SC in the basal state without insulin or glucose infusion, or at an insulin infusion rate of 30.0 mU/kg.min. However, dialysate lactate concentrations in muscle averaged over 45-75 min during the 6.0-mU/kg.min insulin clamp procedure in VR (8.51 +/- 0.71 mg/dl) were significantly (p < 0.05) higher than in SC (6.18 +/- 0.48 mg/dl). These results indicated that insulin action in skeletal muscle and adipose tissue could be evaluated in vivo by using the microdialysis technique, and that an increase in GIR in VR was, in part, explained by an increase in lactate formation in skeletal muscle.
在8只久坐不动的对照大鼠（SC）和7只自主跑步的大鼠（VR）中，研究了体育锻炼对大鼠外周组织中体内胰岛素刺激的葡萄糖利用与糖酵解（乳酸生成）之间关系的影响。我们采用了序贯正常血糖钳夹程序（胰岛素输注速率；6.0、30.0 mU/kg·min），并结合股四头肌、股外侧肌和腹股沟脂肪组织中的微透析技术。在钳夹研究中，在6.0 mU/kg·min胰岛素输注期间45 - 75分钟内平均的葡萄糖输注速率（GIR），VR组（15.36±0.83 mg/kg·min，均值±标准误）显著高于SC组（10.41±0.88 mg/kg·min，p < 0.01），而在30.0 mU/kg·min胰岛素输注期间，VR组和SC组的GIR没有显著差异。在这些组织中，在无胰岛素或葡萄糖输注的基础状态下，以及在胰岛素输注速率为30.0 mU/kg·min时，VR组和SC组的透析液乳酸水平没有显著差异。然而，在VR组6.0 mU/kg·min胰岛素钳夹程序期间45 - 75分钟内肌肉中的透析液乳酸浓度（8.51±0.71 mg/dl）显著高于SC组（6.18±0.48 mg/dl，p < 0.05）。这些结果表明，使用微透析技术可以在体内评估骨骼肌和脂肪组织中的胰岛素作用，并且VR组中GIR的增加部分是由骨骼肌中乳酸生成的增加所解释的。
