未经核糖核酸酶III切割的体外T7核糖核酸翻译。
Translation of T7 RNA in vitro without cleavage by RNase III.
作者信息
Yamada Y, Nakada D
出版信息
J Virol. 1976 Jun;18(3):1155-9. doi: 10.1128/JVI.18.3.1155-1159.1976.
Abstract
T7 early mRNA's are generated from a high-molecular-weight precursor RNA by site-specific RNase III cleavage. When T7 DNA is transcribed in vitro by Escherichia coli RNA polymerase, the transcript is a large, single-piece RNA equivalent to the in vivo precursor RNA. The T7 RNA synthesized in vitro can be translated as a polycistronic messenger without cleavage by RNase III. All T7 early proteins are synthesized in an RNase III-free, protein-synthesizing system directed by the uncleaved T7 RNA.
摘要
T7早期信使核糖核酸（mRNA）是通过位点特异性核糖核酸酶III切割从高分子量前体RNA产生的。当T7 DNA在体外由大肠杆菌RNA聚合酶转录时，转录本是一个大的单链RNA，等同于体内前体RNA。体外合成的T7 RNA可以作为多顺反子信使进行翻译，而无需被核糖核酸酶III切割。所有T7早期蛋白质都是在由未切割的T7 RNA指导的无核糖核酸酶III的蛋白质合成系统中合成的。
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本文引用的文献
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New RNA polymerase from Escherichia coli infected with bacteriophage T7.来自被噬菌体T7感染的大肠杆菌的新型RNA聚合酶。
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Analysis of bacteriophage T7 early RNAs and proteins on slab gels.平板凝胶上噬菌体T7早期RNA和蛋白质的分析。
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5
T7 early messenger RNAs are the direct products of ribonuclease III cleavage.T7早期信使核糖核酸是核糖核酸酶III切割的直接产物。
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6
Early to late switch in bacteriophage T7 development: functional decay of T7 early messenger RNA.噬菌体T7发育过程中的早期到晚期转换：T7早期信使RNA的功能衰退
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7
Isolation of an inhibitor protein of E. coli RNA polymerase from T7 phage infected cell.从T7噬菌体感染的细胞中分离大肠杆菌RNA聚合酶的一种抑制蛋白。
Nature. 1974 Nov 1;252(5478):71-4. doi: 10.1038/252071b0.
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