大肠杆菌的rpoE基因编码σE，对细菌在高温下生长至关重要。
The rpoE gene of Escherichia coli, which encodes sigma E, is essential for bacterial growth at high temperature.
作者信息
Hiratsu K, Amemura M, Nashimoto H, Shinagawa H, Makino K
机构信息
Department of Molecular Microbiology, Osaka University, Japan.
出版信息
J Bacteriol. 1995 May;177(10):2918-22. doi: 10.1128/jb.177.10.2918-2922.1995.
Abstract
In vitro transcription analysis has shown that only RNA polymerase containing an alternative sigma subunit, sigma E, activates transcription from one of the rpoH promoters and the htrA promoter. The location of the rpoE gene encoding sigma E on the Escherichia coli chromosome has recently been established, but no rpoE mutant has yet become available for phenotypic testing. We cloned the rpoE gene from the lambda-ordered clones of the E. coli genome and confirmed that the reconstituted RNA polymerase containing the gene product (E sigma E) can transcribe htrA in vitro. We constructed an rpoE-defective strain by gene disruption using the cloned rpoE gene. We demonstrate that expression of htrA is completely dependent on the rpoE gene in vivo and that the rpoE gene is essential for bacterial growth at high temperature.
摘要
体外转录分析表明，只有含有替代σ亚基σE的RNA聚合酶才能激活rpoH启动子之一和htrA启动子的转录。编码σE的rpoE基因在大肠杆菌染色体上的位置最近已确定，但尚未获得用于表型测试的rpoE突变体。我们从大肠杆菌基因组的λ排序克隆中克隆了rpoE基因，并证实含有该基因产物（EσE）的重组RNA聚合酶能够在体外转录htrA。我们使用克隆的rpoE基因通过基因破坏构建了一个rpoE缺陷菌株。我们证明，在体内htrA的表达完全依赖于rpoE基因，并且rpoE基因对于细菌在高温下生长至关重要。
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