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副嗜沫嗜血杆菌感染:实验室诊断中的一个陷阱。

Haemophilus paraphrophilus infection: a pitfall in laboratory diagnosis.

作者信息

Chadwick P R, Malnick H, Ebizie A O

机构信息

Manchester PHL, Withington Hospital, U.K.

出版信息

J Infect. 1995 Jan;30(1):67-9. doi: 10.1016/s0163-4453(95)92977-0.

DOI:10.1016/s0163-4453(95)92977-0
PMID:7751671
Abstract

Many clinical laboratories have difficulty in identifying a group of organisms which are catalase negative, oxidase positive, Gram negative rods. We describe a case of purulent sacroiliitis due to Haemophilus paraphrophilus where the organism was initially misidentified as Eikenella corrodens leading to inappropriate antimicrobial chemotherapy. We review the strains of H. paraphrophilus and E. corrodens that were identified by the National Collection of Type Cultures over the last ten years. Only 21 of 100 strains identified as E. corrodens were submitted as E. corrodens. Seven strains submitted as possible E. corrodens were identified as H. paraphrophilus. Several different species of Gram negative rods may produce pitting on agar and this seems to be poorly recognised. However, further tests are available to facilitate correct identification of these strains.

摘要

许多临床实验室在鉴定一组过氧化氢酶阴性、氧化酶阳性、革兰氏阴性杆菌的微生物时存在困难。我们描述了一例由副嗜沫嗜血杆菌引起的化脓性骶髂关节炎病例,该微生物最初被误诊为腐蚀艾肯菌,导致了不恰当的抗菌化疗。我们回顾了过去十年中由国家典型培养物保藏中心鉴定的副嗜沫嗜血杆菌和腐蚀艾肯菌菌株。在100株被鉴定为腐蚀艾肯菌的菌株中,只有21株被提交为腐蚀艾肯菌。7株被提交为可能的腐蚀艾肯菌的菌株被鉴定为副嗜沫嗜血杆菌。几种不同种类的革兰氏阴性杆菌可能会在琼脂上产生蚀斑,而这似乎未得到充分认识。然而,可以进行进一步的检测以促进对这些菌株的正确鉴定。

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1
Haemophilus paraphrophilus infection: a pitfall in laboratory diagnosis.副嗜沫嗜血杆菌感染:实验室诊断中的一个陷阱。
J Infect. 1995 Jan;30(1):67-9. doi: 10.1016/s0163-4453(95)92977-0.
2
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引用本文的文献

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Liver and brain abscess caused by Aggregatibacter paraphrophilus in association with a large patent foramen ovale: a case report.副嗜沫聚集杆菌引起的肝脑脓肿合并大型卵圆孔未闭:一例报告
J Med Case Rep. 2010 Feb 24;4:69. doi: 10.1186/1752-1947-4-69.
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Haemophilus paraphrophilus, a rare cause of intracerebral abscess in children.
副嗜沫嗜血杆菌,儿童脑脓肿的罕见病因。
Eur J Pediatr. 2008 Jun;167(6):629-32. doi: 10.1007/s00431-007-0560-6. Epub 2007 Jul 28.
4
Rapid identification of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, and Haemophilus paraphrophilus by restriction enzyme analysis of PCR-amplified 16S rRNA genes.通过对聚合酶链反应扩增的16S rRNA基因进行限制性酶切分析快速鉴定伴放线放线杆菌、嗜沫嗜血杆菌和副嗜沫嗜血杆菌。
J Clin Microbiol. 1997 Jun;35(6):1630-2. doi: 10.1128/jcm.35.6.1630-1632.1997.