Post J, Vooys W C, de Gast G C, Bast B J
Department of Haematology, University Hospital, Utrecht, The Netherlands.
Leuk Res. 1995 Apr;19(4):241-7. doi: 10.1016/0145-2126(94)00142-w.
Before using immunotoxins in vivo, their efficacy is evaluated in in vitro assays. In this study we compare six different assays for the evaluation of immunotoxins: protein and DNA synthesis inhibition assay, chromium release assay, cell line colony assay, limiting dilution assay and clonogenic assay. All assays except the chromium release assay show specificity of the immunotoxins in appropriate concentrations. The protein and DNA synthesis inhibition assays are easy to perform and, therefore, suitable for initial screening, while the clonogenic assay seems to be the best one for immunotoxin efficacy determination.
在体内使用免疫毒素之前，需在体外试验中评估其疗效。在本研究中，我们比较了六种用于评估免疫毒素的不同试验：蛋白质和DNA合成抑制试验、铬释放试验、细胞系集落试验、有限稀释试验和克隆形成试验。除铬释放试验外，所有试验均显示免疫毒素在适当浓度下具有特异性。蛋白质和DNA合成抑制试验易于操作，因此适用于初步筛选，而克隆形成试验似乎是确定免疫毒素疗效的最佳试验。
