Yamamoto N
First Department of Surgery, Chiba University, School of Medicine, Japan.
Nihon Geka Gakkai Zasshi. 1995 Apr;96(4):236-44.
Relationship between nuclear DNA contents and nuclear grade was investigated objectively in 76 invasive ductal breast cancer patients. DNA ploidy and S-phase fraction (SPF) were determined by flow cytometry in fresh frozen specimens. Nuclear grade was evaluated objectively as nuclear area (NA) or coefficiency variant of NA (NA.CV) calculated from morphmeter on H-E stained imprint preparation and needle aspiration smear preparation. NA and NA.CV on each preparation of aneuploid tumors (38) were significantly higher than those of diploid tumors (36). No correlation between NA and SPF was found in both diploid and aneuploid tumors. SPF was related to NA.CV on each preparation only in aneuploid tumors. NA and NA.CV on needle aspiration smear preparation had good relationship to those on H-E stained imprint preparation. These results demonstrated that NA and NA.CV on each preparation reflect DNA contents more objectively and suggested that needle aspiration smear might be useful to estimate the biological malignancies for selection of operative method and decision of neoadjuvant therapy.
对76例浸润性导管癌患者的核DNA含量与核分级之间的关系进行了客观研究。通过流式细胞术测定新鲜冰冻标本中的DNA倍体和S期分数（SPF）。根据苏木精-伊红（H-E）染色压片标本和针吸涂片标本上的形态测量仪计算的核面积（NA）或NA的系数变异（NA.CV），客观评估核分级。非整倍体肿瘤（38例）各标本上的NA和NA.CV显著高于二倍体肿瘤（36例）。在二倍体和非整倍体肿瘤中均未发现NA与SPF之间存在相关性。仅在非整倍体肿瘤中，各标本上的SPF与NA.CV相关。针吸涂片标本上的NA和NA.CV与H-E染色压片标本上的NA和NA.CV具有良好的相关性。这些结果表明，各标本上的NA和NA.CV更客观地反映了DNA含量，并提示针吸涂片可能有助于评估生物学恶性程度，以选择手术方法和决定新辅助治疗。
