Sauer F, Fondell J D, Ohkuma Y, Roeder R G, Jäckle H
Abteilung Molekulare Entwicklungsbiologie, Max-Planck-Institut für Biophysikalische Chemie, Göttingen, Germany.
Nature. 1995 May 11;375(6527):162-4. doi: 10.1038/375162a0.
The zinc-finger protein Krüppel (Kr) is an integral part of the Drosophila segmentation gene cascade and is essential in organogenesis during later embryonic development. In tissue culture, Kr regulates transcription. Monomeric Kr can act as a transcriptional activator, whereas Kr dimers formed at high concentrations cause repression. Here we show that Kr-dependent control of transcription involves functional interactions with components of the basal RNA polymerase II transcription machinery, which includes the initiation factors TFIIA, B, E, F, H and I (refs 10, 11) as well as the TATA-binding protein (TBP) and TBP-associated factors (TAFs) contained in the multisubunit TFIID (ref. 12). Our results indicate that when acting from a site close to a basal promoter, monomeric Kr interacts with TFIIB to activate transcription, whereas an interaction of the Kr dimer with TFIIE beta, a subunit of TFIIE, results in transcriptional repression.
锌指蛋白克虏伯（Kr）是果蝇体节基因级联反应中不可或缺的一部分，在胚胎后期发育的器官形成过程中至关重要。在组织培养中，Kr调控转录。单体Kr可作为转录激活因子，而高浓度形成的Kr二聚体则导致抑制作用。在此我们表明，Kr依赖的转录控制涉及与基础RNA聚合酶II转录机制组件的功能相互作用，该机制包括起始因子TFIIA、B、E、F、H和I（参考文献10、11）以及多亚基TFIID中包含的TATA结合蛋白（TBP）和TBP相关因子（TAFs）（参考文献12）。我们的结果表明，当从靠近基础启动子的位点起作用时，单体Kr与TFIIB相互作用以激活转录，而Kr二聚体与TFIIE的一个亚基TFIIEβ相互作用则导致转录抑制。
