使用新型荧光嵌入剂（吡喃鎓染料P2）快速检测PCR扩增产物 - Suppr

Suppr

超能文献

A rapid detection of PCR amplification product using a new fluorescent intercalator; the pyrylium dye, P2.

作者信息

Yamamoto N, Okamoto T

机构信息

Canon Research Center, Canon Inc., Kanagawa, Japan.

出版信息

Nucleic Acids Res. 1995 Apr 25;23(8):1445-6. doi: 10.1093/nar/23.8.1445.

DOI:10.1093/nar/23.8.1445

PMID:7753640

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC306876/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d531/306876/29a6c7f19a64/nar00008-0175-a.jpg

相似文献

A rapid detection of PCR amplification product using a new fluorescent intercalator; the pyrylium dye, P2.使用新型荧光嵌入剂（吡喃鎓染料P2）快速检测PCR扩增产物

Nucleic Acids Res. 1995 Apr 25;23(8):1445-6. doi: 10.1093/nar/23.8.1445.

Clinical application of a novel DNA fluorescence intercalator, pyrylium dye, for the quantification of PCR products.一种新型DNA荧光嵌入剂——吡喃鎓染料在PCR产物定量中的临床应用。

Scand J Clin Lab Invest. 1996 Nov;56(7):641-7. doi: 10.3109/00365519609090599.

Selective detection of individual DNA molecules by capillary polymerase chain reaction.通过毛细管聚合酶链反应对单个DNA分子进行选择性检测。

Anal Chem. 2001 Apr 1;73(7):1537-43. doi: 10.1021/ac001125p.

Enhanced detection of PCR products through use of TOTO and YOYO intercalating dyes with laser induced fluorescence--capillary electrophoresis.通过使用TOTO和YOYO嵌入染料与激光诱导荧光——毛细管电泳增强PCR产物的检测。

Appl Theor Electrophor. 1993;3(5):235-9.

Screening method for a large quantity of polymerase chain reaction products by measuring YOYO-1 fluorescence on 96-well polypropylene plates.

Anal Biochem. 1994 May 1;218(2):458-9. doi: 10.1006/abio.1994.1207.

Novel intercalators of pyrylium dye into double-stranded DNA.新型吡喃鎓染料插入双链DNA的嵌入剂。

Nucleic Acids Symp Ser. 1993(29):83-4.

Specific triplex binding capacity of mixed base sequence duplex nucleic acids used for single-nucleotide polymorphism detection.用于单核苷酸多态性检测的混合碱基序列双链核酸的特异性三链体结合能力。

Genet Test. 2005 Summer;9(2):111-20. doi: 10.1089/gte.2005.9.111.

Control genes in reverse transcriptase-polymerase chain reaction assays.逆转录聚合酶链反应分析中的对照基因。

Leukemia. 1996 Sep;10(9):1527-8.

Charging YOYO-1 on capillary wall for online DNA intercalation and integrating this approach with multiplex PCR and bare narrow capillary-hydrodynamic chromatography for online DNA analysis.将YOYO-1加载到毛细管壁上进行在线DNA嵌入，并将该方法与多重PCR以及裸窄毛细管-流体动力学色谱法相结合用于在线DNA分析。

Anal Chem. 2015 Feb 3;87(3):1518-22. doi: 10.1021/ac504257b. Epub 2015 Jan 15.

Use of thiazole orange homodimer as an alternative to ethidium bromide for DNA detection in agarose gels.使用噻唑橙同型二聚体替代溴化乙锭用于琼脂糖凝胶中的DNA检测。

Mod Pathol. 1994 Apr;7(3):385-7.

引用本文的文献

Triarylpyrylium-based fluorescent DNA-binding dyes - facile synthesis, substituent effects, and sensing mechanism.基于三芳基吡喃鎓的荧光DNA结合染料——简便合成、取代基效应及传感机制

RSC Adv. 2025 Aug 22;15(36):29952-29959. doi: 10.1039/d5ra04750a. eCollection 2025 Aug 18.

Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications.EvaGreen的特性及其物理化学性质在定量聚合酶链反应（qPCR）应用中的意义

BMC Biotechnol. 2007 Nov 9;7:76. doi: 10.1186/1472-6750-7-76.

A closed tube format for amplification and detection of DNA based on energy transfer.一种基于能量转移的用于DNA扩增和检测的封闭管形式。

Nucleic Acids Res. 1997 Jun 15;25(12):2516-21. doi: 10.1093/nar/25.12.2516.

本文引用的文献

Novel intercalators of pyrylium dye into double-stranded DNA.新型吡喃鎓染料插入双链DNA的嵌入剂。

Nucleic Acids Symp Ser. 1993(29):83-4.

Primary structure of human transferrin receptor deduced from the mRNA sequence.从mRNA序列推导的人转铁蛋白受体的一级结构。

Nature. 1984;311(5987):675-8. doi: 10.1038/311675b0.

A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization.溴化乙锭与核酸之间的荧光复合物。物理化学特性

J Mol Biol. 1967 Jul 14;27(1):87-106. doi: 10.1016/0022-2836(67)90353-1.

Molecular structure of the human cytoplasmic beta-actin gene: interspecies homology of sequences in the introns.人类细胞质β-肌动蛋白基因的分子结构：内含子序列的种间同源性

Proc Natl Acad Sci U S A. 1985 Sep;82(18):6133-7. doi: 10.1073/pnas.82.18.6133.

Stable fluorescent complexes of double-stranded DNA with bis-intercalating asymmetric cyanine dyes: properties and applications.双链DNA与双插入不对称菁染料形成的稳定荧光复合物：性质与应用

Nucleic Acids Res. 1992 Jun 11;20(11):2803-12. doi: 10.1093/nar/20.11.2803.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验