Osbourn S E, Turner A K, Grinsted J
Department of Pathology and Microbiology, University of Bristol, United Kingdom.
Plasmid. 1995 Jan;33(1):65-9. doi: 10.1006/plas.1995.1008.
The DNA sequence of the resolvase gene, resolution sites, and the region between the transposition functions and the end of the mercury resistance operon of the bacterial transposon, Tn3926, is presented. The sequence of Tn3926 upstream of the resolution sites is homologous to that bordering the 11.2-kb insert of Tn21, supporting the idea that this insert transposed into a progenitor of Tn3926. This region of Tn3926 also shows 97.0% identity to the mercury-resistant determinant of the plasmid, pKLH2, suggesting that this plasmid once harbored a close relative of Tn3926. It is proposed that the mercury resistance operon of Tn3926 will have a structure very similar to that found on pKLH2.
本文给出了细菌转座子Tn3926的解离酶基因的DNA序列、解离位点以及转座功能与汞抗性操纵子末端之间区域的序列。解离位点上游的Tn3926序列与Tn21 11.2 kb插入片段边界的序列同源，这支持了该插入片段转座到Tn3926祖先中的观点。Tn3926的这一区域与质粒pKLH2的汞抗性决定簇也有97.0%的同一性，表明该质粒曾携带Tn3926的一个近亲。有人提出，Tn3926的汞抗性操纵子结构将与pKLH2上发现的结构非常相似。
