在轮状病毒SA11和RRV体外产生的重配体中观察到基因片段3的非随机选择和基因片段5的随机选择。

Non-random selection of gene segment 3 and random selection of gene segment 5 observed in reassortants generated in vitro between rotavirus SA11 and RRV.

作者信息

Kobayashi N, Kojima K, Taniguchi K, Urasawa T, Urasawa S

机构信息

Department of Hygiene, School of Medicine, Sapporo Medical University, Japan.

出版信息

Res Virol. 1995 Jan-Feb;146(1):53-9. doi: 10.1016/0923-2516(96)80589-9.

DOI:10.1016/0923-2516(96)80589-9

PMID:7754237

Abstract

A total of 154 clones of reassortants between rotavirus RRV and SA11 were isolated in vitro in order to compare the selection modes of each RNA segment in reassortant formation with those in the in vivo experiment (carried out by Gombold and Ramig) using the same parent strains in which the occurrence of non-random selection of gene segments 3 and 5 was observed. In reassortants isolated from a cross RRV x SA11-L2 (66 isolates) and a cross RRV x SA11-S1 (88 isolates), 36 and 57 different genotypes were identified, respectively. Segregation rates of SA11 RNA segments 1, 5, 8, 9 and 11 were significantly different as compared with those previously reported in the in vivo experiment. While RNA segment 3 was preferentially selected from an SA11 parent, the segregation rate of SA11 gene 5 was relatively low in vitro. Thus, the selection mode of rotavirus RNA segments was found to be different between in vivo and in vitro reassortments.

摘要

为了比较轮状病毒RRV和SA11之间重配体的每个RNA片段在重配体形成中的选择模式与体内实验（由Gombold和Ramig进行）中使用相同亲本菌株时的选择模式（在体内实验中观察到基因片段3和5存在非随机选择），在体外总共分离出154个RRV和SA11之间的重配体克隆。在从RRV×SA11-L2杂交（66个分离株）和RRV×SA11-S1杂交（88个分离株）中分离出的重配体中，分别鉴定出36种和57种不同的基因型。与先前体内实验报道的相比，SA11 RNA片段1、5、8、9和11的分离率有显著差异。虽然RNA片段3优先从SA11亲本中选择，但SA11基因5在体外的分离率相对较低。因此，发现轮状病毒RNA片段的选择模式在体内和体外重配中有所不同。