Matyshevskaia O P, Slatvinskaia E A, Kucherenko N E
Ukr Biokhim Zh (1978). 1994 May-Jun;66(3):60-6.
After spleen lymphocytes incubation with 14C-arachidonic acid 14C label was found associated predominantly with phosphatidylethanolamine (PE) and phosphatidylcholine (PC). Addition of 0.5 mkM a 23187 to the incubation media with 2 mM CaCl2 caused a 4-fold increase in release of labeled free arachidonate in lymphocytes of the control animals. A dose-dependent increase in release of free arachidonate and membrane Ca(2+)-dependent phospholipase activity towards exogenous 2-14C-arachidonoyl-PE and -PC take place in a period 6-12 h after animal's irradiation in a doses 0.5 and 1.0 Gy. The maximum arachidonate release in 6h following 1 Gy irradiation correlates with activation of more active phospholipase A2, hydrolyzing PE. Free arachidonate is supposed to be mobilized from different phospholipid pools, depending on the content of Ca2+ in the cells after irradiation.
用¹⁴C - 花生四烯酸孵育脾淋巴细胞后，发现¹⁴C标记主要与磷脂酰乙醇胺（PE）和磷脂酰胆碱（PC）相关。在含有2 mM氯化钙的孵育培养基中加入0.5 μM离子载体A23187，可使对照动物淋巴细胞中标记的游离花生四烯酸释放增加4倍。在动物接受0.5和1.0 Gy剂量照射后的6 - 12小时内，游离花生四烯酸的释放呈剂量依赖性增加，且膜Ca²⁺依赖性磷脂酶对外源² - ¹⁴C - 花生四烯酰 - PE和 - PC的活性也增加。1 Gy照射后6小时内花生四烯酸的最大释放与更具活性的磷脂酶A2的激活相关，该酶可水解PE。游离花生四烯酸被认为是从不同的磷脂池中动员出来的，这取决于照射后细胞内Ca²⁺的含量。
