Gomez H F, Johnson R, Guven H, McKinney P, Phillips S, Judson F, Brent J
Rocky Mountain Poison and Drug Center, Denver General Hospital, Denver Health and Hospitals, University of Colorado Health Sciences Center, USA.
Ann Emerg Med. 1995 Jun;25(6):818-22. doi: 10.1016/s0196-0644(95)70214-8.
We evaluated the effectiveness of activated charcoal (AC) in adsorbing Clostridium botulinum type A toxin using a mouse bioassay.
Prospective, blinded, randomized, controlled animal study.
Animal care facility.
One hundred forty Swiss/Webster ND-4 strain mice.
Food contaminated with type A botulinum toxin was homogenized in a phosphate/gel buffer (pH 6.2). The concentrate was diluted by factors of 1:10, 1:50, and 1:100. AC was added to aliquots of the dilutions to a 20% final concentration. The samples were centrifuged, supernatant was removed, and separate groups of mice were injected intraperitoneally with .5 mL of each dilution (those treated with AC and controls untreated with AC). The animals were then observed over 5 days for signs of botulism.
None of the 60 animals injected intraperitoneally with dilutions treated with AC was observed to have any signs of botulism. In contrast, deaths were observed in 10 of 20, 9 of 20 and 4 of 20 mice injected with untreated dilutions of 1:100, 1:50, and 1:10, respectively (P < .004).
In this model, treatment of botulinum toxin with AC before administration resulted in greatly reduced morbidity and mortality.
我们使用小鼠生物测定法评估了活性炭（AC）对A型肉毒杆菌毒素的吸附效果。
前瞻性、盲法、随机、对照动物研究。
动物护理设施。
140只瑞士/韦伯斯特ND - 4品系小鼠。
将被A型肉毒杆菌毒素污染的食物在磷酸盐/凝胶缓冲液（pH 6.2）中匀浆。浓缩液按1:10、1:50和1:100的比例稀释。向各稀释液的等分试样中加入AC，使其最终浓度达到20%。将样品离心，去除上清液，然后将不同组的小鼠腹腔注射0.5 mL每种稀释液（用AC处理的和未用AC处理的对照）。然后对动物观察5天，观察肉毒中毒的迹象。
腹腔注射经AC处理的稀释液的60只动物中，未观察到任何肉毒中毒迹象。相比之下，分别注射未经处理的1:100、1:50和1:10稀释液的20只小鼠中，有10只、9只和4只死亡（P < 0.004）。
在该模型中，给药前用AC处理肉毒杆菌毒素可大大降低发病率和死亡率。
