Schirmbeck R, Reimann J
Abteilung Bakteriologie, Universität Ulm, Donau, Germany.
Behring Inst Mitt. 1994 Dec(95):14-22.
CD8+ cytotoxic T lymphocytes (CTL) mediate protective effector functions against many viral, bacterial and parasitic infections. CTL specifically recognize peptides presented by major histocompatibility complex (MHC) class I molecules on the surface of cells. Usually, CTL are stimulated by peptides from intracellularly synthesized, 'endogenous' protein antigens processed in the cytosol or endoplasmic reticulum. Because of this 'endogenous' processing pathway, immunization with 'exogenous' proteins rarely induces class I-restricted CTL responses. We have found that immunization of mice without adjuvants with a single low dose of, either different lipoprotein particles, or various denatured protein antigens by various routes efficiently primes class I-restricted CTL responses of defined epitope and restriction specificity. Priming of CTL requires processing of S-protein and is not inducible by the respective immunogenic peptides. These data reveal a novel immunogenic property of these two types of 'exogenous' protein antigens. The observation is relevant for the development of subunit vaccines designed to specifically stimulate T cell responses.
CD8 + 细胞毒性T淋巴细胞（CTL）介导针对多种病毒、细菌和寄生虫感染的保护性效应功能。CTL特异性识别细胞表面主要组织相容性复合体（MHC）I类分子呈递的肽段。通常，CTL由在胞质溶胶或内质网中加工的细胞内合成的“内源性”蛋白质抗原的肽段刺激。由于这种“内源性”加工途径，用“外源性”蛋白质免疫很少诱导I类限制性CTL反应。我们发现，通过各种途径用单一低剂量的不同脂蛋白颗粒或各种变性蛋白质抗原对无佐剂小鼠进行免疫，可有效引发具有明确表位和限制性特异性的I类限制性CTL反应。CTL的引发需要S蛋白的加工，且相应的免疫原性肽不能诱导。这些数据揭示了这两种“外源性”蛋白质抗原的一种新的免疫原性特性。该观察结果与旨在特异性刺激T细胞反应的亚单位疫苗的开发相关。
