Konneh M K, Rutherford C, Li S R, Anggård E E, Ferns G A
William Harvey Research Institute, St. Bartholomew's Hospital Medical College, University of London, UK.
Atherosclerosis. 1995 Feb;113(1):29-39. doi: 10.1016/0021-9150(94)05423-g.
We have investigated the effect of the naturally occurring, lipid-soluble antioxidant, vitamin E, on the intimal response to balloon injury in the cholesterol-fed rat. We found that in animals receiving a 0.5% vitamin E plus 1% cholesterol diet, neo-intimal thickening was reduced by 30% (P < 0.025) compared to animals receiving either cholesterol alone, or a control chow diet. In all three dietary groups, the intimal lesion consisted predominantly of smooth muscle cells, and few monocytes/macrophages (< 0.5%) could be identified by staining with the monoclonal antibody ED-1. In vitro, vitamin E inhibited platelet-derived growth factor- (PDGF) (20 ng/ml) and serum (2%)-induced mitogenesis of both adult rat thoracic aortic smooth muscle cells and an embryonic rat aortic smooth muscle cell line (A7r5), dose-dependently. These data suggest that reactive oxygen species may be involved in the intimal response to balloon catheter injury, and that antioxidants, such as vitamin E, may offer some protection against restenosis. Although the way by which it does so is unclear, one possible mechanism is by a direct inhibitory effect on the accumulation of smooth muscle cells within the developing neo-intima.
我们研究了天然存在的脂溶性抗氧化剂维生素E对喂食胆固醇的大鼠球囊损伤内膜反应的影响。我们发现，与单独喂食胆固醇或对照普通饲料的动物相比，接受0.5%维生素E加1%胆固醇饮食的动物新生内膜增厚减少了30%（P<0.025）。在所有三个饮食组中，内膜病变主要由平滑肌细胞组成，用单克隆抗体ED-1染色几乎无法识别单核细胞/巨噬细胞（<0.5%）。在体外，维生素E剂量依赖性地抑制血小板衍生生长因子（PDGF）（20 ng/ml）和血清（2%）诱导的成年大鼠胸主动脉平滑肌细胞和胚胎大鼠主动脉平滑肌细胞系（A7r5）的有丝分裂。这些数据表明，活性氧可能参与了球囊导管损伤的内膜反应，而抗氧化剂，如维生素E，可能对再狭窄提供一些保护。虽然其作用方式尚不清楚，但一种可能的机制是对新生内膜中平滑肌细胞的积累产生直接抑制作用。
