Paulsen J, Ochs D, Harder M, Duvos C, Mayer H, Wingender E
Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany.
J Biotechnol. 1995 Apr 15;39(2):129-36. doi: 10.1016/0168-1656(95)00002-8.
Human parathyroid hormone (hPTH) has been bacterially expressed in bioreactors as cro-beta-galactosidase-hPTH fusion protein. We have developed a large-scale purification scheme that exploits the pH-dependent differential solubility of hPTH and a two-step chromatographic procedure. We demonstrate that in a number of assay systems, the recombinant material obtained by this procedure is biologically active.
