Boyce R W, Wronski T J, Ebert D C, Stevens M L, Paddock C L, Youngs T A, Gundersen H J
Procter and Gamble Pharmaceuticals, Norwich, NY, USA.
Bone. 1995 Feb;16(2):209-13. doi: 10.1016/8756-3282(94)00031-t.
Newly developed unbiased stereological methods were employed to investigate the effects of estrogen deficiency on the three-dimensional connectivity of vertebral cancellous bone from ovariectomized (OVX) rats. The effects of two classes of antiresorptive agents, estrogen and bisphosphonates, on changes in connectivity in this animal model were also evaluated. Female rats were either sham-operated (sham-op) or surgically OVX at 90 days of age. OVX rats were administered either vehicle, estrogen (10 micrograms/kg 17-beta estradiol, 5 days/week subcutaneously [SC], etidronate disodium (5 mg/kg SC) or risedronate (5 micrograms/kg SC). The bisphosphonates were administered daily for 1 week followed by 3 weeks with no treatment. Treatment duration was 360 days. Systematic random sections, 30-microns thick, were prepared from methylmethacrylate-embedded decalcified second lumbar vertebrae. Total trabecular number and connectivity density were estimated using the ConnEulor principle. Vertebral cancellous bone volume was estimated on undecalcified sections from the first lumbar vertebrae. Connectivity density and cancellous bone volume were significantly reduced (approximately 25% and 40%, respectively) in the OVX group compared with the sham-op group. Estrogen treatment essentially maintained connectivity and cancellous bone volume at the level of the sham-op rats. Connectivity density and total trabecular number were significantly increased in the etidronate- and risedronate-treated rats compared with both the sham-op and OVX rats. These data demonstrate that reduction in the three-dimensional connectivity of vertebral cancellous bone is a long-term consequence of ovariectomy in the rat. This reduction in connectivity can be effectively prevented by the administration of antiresorptive agents such as estrogen, etidronate and risedronate.(ABSTRACT TRUNCATED AT 250 WORDS)
采用新开发的无偏体视学方法，研究雌激素缺乏对去卵巢（OVX）大鼠椎骨松质骨三维连通性的影响。还评估了两类抗吸收剂，即雌激素和双膦酸盐，对该动物模型连通性变化的影响。雌性大鼠在90日龄时接受假手术（假手术组）或手术去卵巢。OVX大鼠分别给予溶剂、雌激素（10微克/千克17-β雌二醇，每周皮下注射5天）、依替膦酸二钠（5毫克/千克皮下注射）或利塞膦酸盐（5微克/千克皮下注射）。双膦酸盐每日给药1周，随后3周不给药。治疗持续时间为360天。从甲基丙烯酸甲酯包埋的脱钙第二腰椎制备30微米厚的系统随机切片。使用ConnEulor原理估计总小梁数量和连通性密度。从第一腰椎的未脱钙切片估计椎骨松质骨体积。与假手术组相比，OVX组的连通性密度和松质骨体积显著降低（分别约为25%和40%）。雌激素治疗基本将连通性和松质骨体积维持在假手术大鼠的水平。与假手术组和OVX组相比，依替膦酸和利塞膦酸盐治疗的大鼠的连通性密度和总小梁数量显著增加。这些数据表明，椎骨松质骨三维连通性的降低是大鼠去卵巢的长期后果。通过给予抗吸收剂如雌激素、依替膦酸和利塞膦酸盐，可以有效预防连通性的这种降低。（摘要截断于250字）
