蜂毒肽对二棕榈酰磷脂酰胆碱-胆固醇液晶相的作用：一项固态2H和31P核磁共振研究。
Action of melittin on the DPPC-cholesterol liquid-ordered phase: a solid state 2H-and 31P-NMR study.
作者信息
Pott T, Dufourc E J
机构信息
Centre de Recherche Paul Pascal, Centre National de la Recherche Scientifique, Pessac, France.
出版信息
Biophys J. 1995 Mar;68(3):965-77. doi: 10.1016/S0006-3495(95)80272-9.
Abstract
Solid-state deuterium and phosphorus-31 nuclear magnetic resonance studies of deuterium-labeled beta--[2,2',3,4,4',6-2H6]-cholesterol and 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine have been undertaken to monitor the action of melittin on model membranes containing 30 mol% cholesterol, both at the molecular and macroscopic level. Cholesterol totally inhibits the toxin-triggered formation of large unilamellar vesicles and strongly restricts the appearance of small discs. The latter remain stable over a wide temperature range (20-60 degrees C) because of an increase in their cholesterol content as the temperature increases. This process is related to a constant disc hydrophobic thickness of approximately 29 A. The system, when not in the form of discs, appears to be composed of very large vesicles on which melittin promotes magnetically induced ellipsoidal deformation. This deformation is the greatest when the maximum of discs is observed. A model to describe both the disc formation and stability is proposed.
摘要
已开展了对氘标记的β-[2,2',3,4,4',6-2H6]-胆固醇和1,2-二棕榈酰-sn-甘油-3-磷脂酰胆碱的固态氘和磷-31核磁共振研究，以在分子和宏观层面监测蜂毒素对含30摩尔%胆固醇的模型膜的作用。胆固醇完全抑制毒素触发的大单层囊泡的形成，并强烈限制小圆盘的出现。由于温度升高时其胆固醇含量增加，后者在很宽的温度范围（20-60摄氏度）内保持稳定。此过程与约29埃的恒定圆盘疏水厚度有关。当该系统不是圆盘形式时，似乎由非常大的囊泡组成，蜂毒素会促使其发生磁诱导的椭球变形。当观察到圆盘数量最多时，这种变形最大。提出了一个描述圆盘形成和稳定性的模型。
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