Mi L, Jin B, Cui Y, Gao L, Xu Z
Department of Immunology, Fourth Military Medical University, Xi'an, Shaanxi, China.
Hum Antibodies Hybridomas. 1994;5(3-4):187-90.
A human hybridoma cell line C8 secreting human monoclonal anti-idiotypic antibodies (alpha Id) was established by fusing Epstein-Barr virus transfected peripheral blood lymphocytes from a hemorrhagic fever with renal syndrome (HFRS) patient with interspecific myeloma SHM-D33 cells. The alpha ID human monoclonal antibody (hMAb) could react with anti-HFRSV Ab1 from different species, and the reaction could be inhibited by HFRSV antigen, indicating that anti-idiotype hMAb bears the internal image of HFRSV antigen. Anti-HFRSV antibodies (Ab3) with neutralizing and hemagglutination inhibition activities in BALB/c mice or rabbits induced by the alpha Id h MAb were produced, suggesting that the internal image beared by anti-idiotype antibodies may be related to neutralizing and hemagglutinating epitopes on HFRSV antigen.
通过将一名肾综合征出血热（HFRS）患者经爱泼斯坦 - 巴尔病毒转染的外周血淋巴细胞与种间骨髓瘤SHM - D33细胞融合，建立了分泌人抗独特型单克隆抗体（αId）的人杂交瘤细胞系C8。该αId人单克隆抗体（hMAb）可与来自不同物种的抗HFRSV Ab1发生反应，且该反应可被HFRSV抗原抑制，表明抗独特型hMAb具有HFRSV抗原的内影像。由αId hMAb诱导产生的在BALB/c小鼠或兔体内具有中和及血凝抑制活性的抗HFRSV抗体（Ab3），提示抗独特型抗体所具有的内影像可能与HFRSV抗原上的中和及血凝表位有关。
