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Liquid chromatography/mass spectrometry with collision-induced dissociation of arachidonic acid metabolites derivatized with aminobenzenesulphonic acid.

作者信息

Yang Y, Griffiths W J, Lindgren J A, Sjövall J

机构信息

Department of Medical Biochemistry & Biophysics, Karolinska Institutet, Stockholm, Sweden.

出版信息

Rapid Commun Mass Spectrom. 1995;9(4):289-99. doi: 10.1002/rcm.1290090407.

DOI:10.1002/rcm.1290090407
PMID:7756703
Abstract

A fused-silica capillary high-performance liquid chromatography column (i.d. 0.25 mm) packed with octadecylsilane-bonded silica has been used on-line with continuous-flow negative-ion fast-atom bombardment (FAB) mass spectrometry for the separation and structural analysis of series of fatty acids and arachidonic acid metabolites derivatized with 4-aminobenzenesulphonic acid. Negative-ion FAB spectra of the derivatized lipids showed strong [M-H] pseudomolecular ions. Collision-induced dissociation of these anions produced structurally informative daughter ions, arising from charge-remote fragmentations.

摘要

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