García Reverte J M, Bernabeu Esclapez A, Muñoz Ramos J, Vicente Ortega V, Canteras Jordana M
Department of Pathology, Faculty of Medicine, University of Murcia, Spain.
Histol Histopathol. 1995 Jan;10(1):79-84.
An established human melanoma cell line was treated with several concentrations of three antineoplastic drugs: melphalan (0.016, 0.032, 0.16 microns), CCNU (1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea) (0.04, 0.21, 0.42 microM), and 4-OHA (4-hydroxyanisole) (4.01 x 10(-4), 1.20 x 10(-3), 2.4 x 10(-3) microM), and the effects on cell growth and viability were compared. 24 hours after treatment, 4-OHA (ID50 = 2.4 x 10(-3) microM) was more cytotoxic than melphalan (ID50 = 0.016 microM) and CCNU (ID50 = 0.21 microM). However, after 96 hours exposure, the most effective drug was CCNU (growth rate = -1.277), which caused the death of the culture. This was followed by melphalan (growth rate = -1.024) and finally 4-OHA (growth rate = -0.69). Similar ultrastructural cell injuries were observed after the use of the three drugs: the dilation of endoplasmic reticulum vesicles and the nuclear membrane; mitochondria swelling; and the existence of lamellar structures and cytoplasmic vacuoles.
美法仑（0.016、0.032、0.16微摩尔）、洛莫司汀（1-(2-氯乙基)-3-环己基-1-亚硝基脲）（0.04、0.21、0.42微摩尔）和4-羟基茴香醚（4.01×10⁻⁴、1.20×10⁻³、2.4×10⁻³微摩尔），并比较对细胞生长和活力的影响。处理24小时后，4-羟基茴香醚（半数抑制浓度=2.4×10⁻³微摩尔）比美法仑（半数抑制浓度=0.016微摩尔）和洛莫司汀（半数抑制浓度=0.21微摩尔）更具细胞毒性。然而，暴露96小时后，最有效的药物是洛莫司汀（生长率=-1.277），它导致培养物死亡。其次是美法仑（生长率=-1.024），最后是4-羟基茴香醚（生长率=-0.69）。使用这三种药物后观察到类似的超微结构细胞损伤：内质网小泡和核膜扩张；线粒体肿胀；以及存在层状结构和细胞质空泡。
