Roybal J E, Pfenning A P, Munns R K, Holland D C, Hurlbut J A, Long A R
U.S. Food and Drug Administration, Animal Drugs Research Center, Denver Federal Center, CO 80225-0087, USA.
J AOAC Int. 1995 Mar-Apr;78(2):453-7.
To determine residues of malachite green (MG) and its metabolite, leucomalachite green (LMG), in catfish tissue, analytes are extracted with acetonitrile-buffer and the extract is partitioned into methylene chloride. Final cleanup and isolation are performed on neutral alumina solid-phase extraction (SPE) and propylsulfonic acid cation-exchange SPE columns before analysis by liquid chromatography with visible detection. PbO2 postcolumn oxidation is performed by isocratic elution with a buffered mobile phase from a cyano column. Recoveries and relative standard deviations (RSDs) from fortified catfish tissues were 72.9% (RSD, 1.92%; 23 ppb), 75.5% (RSD, 6.85%; 11 ppb), and 69.6% (RSD, 6.93%; 5.7 ppb) for MG and 87.4% (RSD, 2.92%; 21 ppb), 88.1% (RSD, 5.94%; 10 ppb), and 82.6% (RSD, 11.5%; 5.3 ppb) for LMG. The method was applied to MG-incurred catfish at depuration times of 0, 2, 4, 8, and 24 h. Average levels of residual MG and LMG in the 24 h depuration catfish tissue were 73.4 and 289 ppb, respectively.
为测定鲶鱼组织中孔雀石绿（MG）及其代谢物隐色孔雀石绿（LMG）的残留量，用乙腈 - 缓冲液提取分析物，提取物再用二氯甲烷进行液液分配。在通过可见检测的液相色谱分析之前，最终的净化和分离在中性氧化铝固相萃取（SPE）柱和丙基磺酸阳离子交换SPE柱上进行。通过氰基柱用缓冲流动相进行等度洗脱来进行柱后PbO₂氧化。在添加了MG和LMG的鲶鱼组织中的回收率和相对标准偏差（RSD），MG分别为72.9%（RSD，1.92%；23 ppb）、75.5%（RSD，6.85%；11 ppb）和69.6%（RSD，6.93%；5.7 ppb），LMG分别为87.4%（RSD，2.92%；21 ppb）、88.1%（RSD，5.94%；10 ppb）和82.6%（RSD，11.5%；5.3 ppb）。该方法应用于净化时间为0、2、4、8和24小时的添加了MG的鲶鱼。在净化24小时的鲶鱼组织中，MG和LMG的平均残留水平分别为73.4和289 ppb。
