Boison J O, Keng L J
Agriculture and Agri-Food Canada, Health of Animals Laboratory, Saskatoon, SK.
J AOAC Int. 1995 May-Jun;78(3):651-8.
A simple, sensitive, and rapid method for simultaneous determination of sulfamethazine and sulfadimethoxine residues in animal tissues by liquid chromatography (LC) with on-line confirmation by thermospray mass spectrometry is reported. Tissue extracts, after cleanup on C18 cartridges, were injected into a reversed-phase C18 column, and the sulfa drugs were determined by ultraviolet (UV) detection at 265 nm. On-line confirmation of sulfamethazine and sulfadimethoxine in the extracts by mass spectrometry was obtained by feeding the sulfa drugs eluting from the chromatographic column after UV detection directly into the ion source of a mass spectrometer with a thermospray ionization LC interface. Analytical results obtained with the LC method, which has detection limits of 2 and 10 ng/g of tissue, for sulfamethazine and sulfadimethoxine, respectively, compared favorably with those obtained with the official thin-layer chromatographic-densitometric method.
报道了一种通过液相色谱（LC）同时测定动物组织中磺胺二甲嘧啶和磺胺二甲氧嘧啶残留量的简单、灵敏且快速的方法，该方法通过热喷雾质谱进行在线确证。组织提取物在C18柱上净化后，注入反相C18柱，磺胺类药物通过在265nm处的紫外（UV）检测进行测定。通过将紫外检测后从色谱柱洗脱的磺胺类药物直接送入带有热喷雾电离LC接口的质谱仪的离子源，对提取物中的磺胺二甲嘧啶和磺胺二甲氧嘧啶进行质谱在线确证。液相色谱法对磺胺二甲嘧啶和磺胺二甲氧嘧啶的检测限分别为2和10 ng/g组织，与官方薄层色谱 - 光密度法得到的分析结果相比具有优势。
